Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat

Detalhes bibliográficos
Autor(a) principal: Schneid,Andréa dos Santos
Data de Publicação: 2006
Outros Autores: Rodrigues,Kelly Lameiro, Chemello,Davi, Tondo,Eduardo Cesar, Ayub,Marco Antônio Zacchia, Aleixo,José Antonio Guimarães
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027
Resumo: In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-.
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spelling Evaluation of an indirect ELISA for the detection of Salmonella in chicken meatSalmonellamonoclonal antibodypoliclonal antibodyELISAIn this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-.Sociedade Brasileira de Microbiologia2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027Brazilian Journal of Microbiology v.37 n.3 2006reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822006000300027info:eu-repo/semantics/openAccessSchneid,Andréa dos SantosRodrigues,Kelly LameiroChemello,DaviTondo,Eduardo CesarAyub,Marco Antônio ZacchiaAleixo,José Antonio Guimarãeseng2006-12-18T00:00:00Zoai:scielo:S1517-83822006000300027Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-12-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
title Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
spellingShingle Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
Schneid,Andréa dos Santos
Salmonella
monoclonal antibody
policlonal antibody
ELISA
title_short Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
title_full Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
title_fullStr Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
title_full_unstemmed Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
title_sort Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
author Schneid,Andréa dos Santos
author_facet Schneid,Andréa dos Santos
Rodrigues,Kelly Lameiro
Chemello,Davi
Tondo,Eduardo Cesar
Ayub,Marco Antônio Zacchia
Aleixo,José Antonio Guimarães
author_role author
author2 Rodrigues,Kelly Lameiro
Chemello,Davi
Tondo,Eduardo Cesar
Ayub,Marco Antônio Zacchia
Aleixo,José Antonio Guimarães
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Schneid,Andréa dos Santos
Rodrigues,Kelly Lameiro
Chemello,Davi
Tondo,Eduardo Cesar
Ayub,Marco Antônio Zacchia
Aleixo,José Antonio Guimarães
dc.subject.por.fl_str_mv Salmonella
monoclonal antibody
policlonal antibody
ELISA
topic Salmonella
monoclonal antibody
policlonal antibody
ELISA
description In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822006000300027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.37 n.3 2006
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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