Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level

Detalhes bibliográficos
Autor(a) principal: Delamare,Ana Paula Longaray
Data de Publicação: 2002
Outros Autores: Artico,Liane de Oliveira, Grazziotin,Felipe Gobbi, Echeverrigaray,Sergio, Costa,Sérgio Olavo Pinto da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016
Resumo: Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but the high variability limits its potential as an aiding method for species identification.
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spelling Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species levelAeromonasmolecular markersspecies identificationFifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but the high variability limits its potential as an aiding method for species identification.Sociedade Brasileira de Microbiologia2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016Brazilian Journal of Microbiology v.33 n.4 2002reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822002000400016info:eu-repo/semantics/openAccessDelamare,Ana Paula LongarayArtico,Liane de OliveiraGrazziotin,Felipe GobbiEcheverrigaray,SergioCosta,Sérgio Olavo Pinto daeng2014-04-28T00:00:00Zoai:scielo:S1517-83822002000400016Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-04-28T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
title Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
spellingShingle Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
Delamare,Ana Paula Longaray
Aeromonas
molecular markers
species identification
title_short Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
title_full Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
title_fullStr Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
title_full_unstemmed Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
title_sort Total protein electrophoresis and RAPD fingerprinting analysis for the identification of Aeromonas at the species level
author Delamare,Ana Paula Longaray
author_facet Delamare,Ana Paula Longaray
Artico,Liane de Oliveira
Grazziotin,Felipe Gobbi
Echeverrigaray,Sergio
Costa,Sérgio Olavo Pinto da
author_role author
author2 Artico,Liane de Oliveira
Grazziotin,Felipe Gobbi
Echeverrigaray,Sergio
Costa,Sérgio Olavo Pinto da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Delamare,Ana Paula Longaray
Artico,Liane de Oliveira
Grazziotin,Felipe Gobbi
Echeverrigaray,Sergio
Costa,Sérgio Olavo Pinto da
dc.subject.por.fl_str_mv Aeromonas
molecular markers
species identification
topic Aeromonas
molecular markers
species identification
description Fifteen well-defined strains of Aeromonas of thirteen species were analyzed by SDS protein electrophoretic analysis (SDS-PAGE) and random amplified polymorphic DNA analysis (RAPD). The comparison between the patterns obtained by both methods allowed differentiating all the strains. Clusters formed by the unweighted pair group method with arithmetic averages applied to protein data correlates with the genetic and biochemical information about the species. The results show that protein fingerprinting has the potential to differentiate Aeromonas species, but the low qualitative variation indicates that this technique is not efficient for the characterization of strains within a species. Conversely, RAPD fingerprinting allows the identification of strains but the high variability limits its potential as an aiding method for species identification.
publishDate 2002
dc.date.none.fl_str_mv 2002-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822002000400016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.33 n.4 2002
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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