Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach

Detalhes bibliográficos
Autor(a) principal: Shabbiri,Khadija
Data de Publicação: 2012
Outros Autores: Adnan,Ahmad, Jamil,Sania, Ahmad,Waqar, Noor,Bushra, Rafique,H.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031
Resumo: Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
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spelling Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approachBrevibacterium linensProteaseSolid-state fermentationPlackett-Burman DesignCentral Composite Design (CCD)Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.Sociedade Brasileira de Microbiologia2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031Brazilian Journal of Microbiology v.43 n.3 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000300031info:eu-repo/semantics/openAccessShabbiri,KhadijaAdnan,AhmadJamil,SaniaAhmad,WaqarNoor,BushraRafique,H.M.eng2012-11-26T00:00:00Zoai:scielo:S1517-83822012000300031Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-11-26T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
spellingShingle Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
Shabbiri,Khadija
Brevibacterium linens
Protease
Solid-state fermentation
Plackett-Burman Design
Central Composite Design (CCD)
title_short Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_full Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_fullStr Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_full_unstemmed Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_sort Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
author Shabbiri,Khadija
author_facet Shabbiri,Khadija
Adnan,Ahmad
Jamil,Sania
Ahmad,Waqar
Noor,Bushra
Rafique,H.M.
author_role author
author2 Adnan,Ahmad
Jamil,Sania
Ahmad,Waqar
Noor,Bushra
Rafique,H.M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Shabbiri,Khadija
Adnan,Ahmad
Jamil,Sania
Ahmad,Waqar
Noor,Bushra
Rafique,H.M.
dc.subject.por.fl_str_mv Brevibacterium linens
Protease
Solid-state fermentation
Plackett-Burman Design
Central Composite Design (CCD)
topic Brevibacterium linens
Protease
Solid-state fermentation
Plackett-Burman Design
Central Composite Design (CCD)
description Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822012000300031
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.43 n.3 2012
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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