Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031 |
Resumo: | Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium. |
id |
SBM-1_0c19914cdd202bf56123ad156b0a96c2 |
---|---|
oai_identifier_str |
oai:scielo:S1517-83822012000300031 |
network_acronym_str |
SBM-1 |
network_name_str |
Brazilian Journal of Microbiology |
repository_id_str |
|
spelling |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approachBrevibacterium linensProteaseSolid-state fermentationPlackett-Burman DesignCentral Composite Design (CCD)Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.Sociedade Brasileira de Microbiologia2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031Brazilian Journal of Microbiology v.43 n.3 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000300031info:eu-repo/semantics/openAccessShabbiri,KhadijaAdnan,AhmadJamil,SaniaAhmad,WaqarNoor,BushraRafique,H.M.eng2012-11-26T00:00:00Zoai:scielo:S1517-83822012000300031Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-11-26T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach |
title |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach |
spellingShingle |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach Shabbiri,Khadija Brevibacterium linens Protease Solid-state fermentation Plackett-Burman Design Central Composite Design (CCD) |
title_short |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach |
title_full |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach |
title_fullStr |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach |
title_full_unstemmed |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach |
title_sort |
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach |
author |
Shabbiri,Khadija |
author_facet |
Shabbiri,Khadija Adnan,Ahmad Jamil,Sania Ahmad,Waqar Noor,Bushra Rafique,H.M. |
author_role |
author |
author2 |
Adnan,Ahmad Jamil,Sania Ahmad,Waqar Noor,Bushra Rafique,H.M. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Shabbiri,Khadija Adnan,Ahmad Jamil,Sania Ahmad,Waqar Noor,Bushra Rafique,H.M. |
dc.subject.por.fl_str_mv |
Brevibacterium linens Protease Solid-state fermentation Plackett-Burman Design Central Composite Design (CCD) |
topic |
Brevibacterium linens Protease Solid-state fermentation Plackett-Burman Design Central Composite Design (CCD) |
description |
Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822012000300031 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.43 n.3 2012 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122204722561024 |