In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates

Detalhes bibliográficos
Autor(a) principal: El-Nakeeb,Moustafa A
Data de Publicação: 2011
Outros Autores: Abou-Shleib,Hamida M, Khalil,Amal M, Omar,Hoda G, El-Halfawy,Omar M
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300018
Resumo: Antihistaminics are widely used for various indications during microbial infection. Hence, this paper investigates the antimicrobial activities of 10 antihistaminics belonging to both old and new generations using multiresistant Gram-positive and Gram-negative clinical isolates. The bacteriostatic activity of antihistaminics was investigated by determining their MIC both by broth and agar dilution techniques against 29 bacterial strains. Azelastine, cyproheptadine, mequitazine and promethazine were the most active among the tested drugs. Diphenhydramine and cetirizine possessed weaker activity whereas doxylamine, fexofenadine and loratadine were inactive even at the highest tested concentration (1 mg/ml). The MIC of meclozine could not be determined as it precipitated with the used culture media. The MBC values of antihistaminics were almost identical to the corresponding MIC values. The bactericidal activity of antihistaminics was also studied by the viable count technique in sterile saline solution. Evident killing effects were exerted by mequitazine, meclozine, azelastine and cyproheptadine. Moreover, the dynamics of bactericidal activity of azelastine were studied by the viable count technique in nutrient broth. This activity was found to be concentration-dependant. This effect was reduced on increasing the inoculum size while it was increased on raising the pH. The post-antimicrobial effect of 100 fg/ml azelastine was also determined and reached up to 3.36 h.
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spelling In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolatesAntihistaminicsbactericidal activitybacteriostatic activityGram-negative isolatesGram-positive isolatesAntihistaminics are widely used for various indications during microbial infection. Hence, this paper investigates the antimicrobial activities of 10 antihistaminics belonging to both old and new generations using multiresistant Gram-positive and Gram-negative clinical isolates. The bacteriostatic activity of antihistaminics was investigated by determining their MIC both by broth and agar dilution techniques against 29 bacterial strains. Azelastine, cyproheptadine, mequitazine and promethazine were the most active among the tested drugs. Diphenhydramine and cetirizine possessed weaker activity whereas doxylamine, fexofenadine and loratadine were inactive even at the highest tested concentration (1 mg/ml). The MIC of meclozine could not be determined as it precipitated with the used culture media. The MBC values of antihistaminics were almost identical to the corresponding MIC values. The bactericidal activity of antihistaminics was also studied by the viable count technique in sterile saline solution. Evident killing effects were exerted by mequitazine, meclozine, azelastine and cyproheptadine. Moreover, the dynamics of bactericidal activity of azelastine were studied by the viable count technique in nutrient broth. This activity was found to be concentration-dependant. This effect was reduced on increasing the inoculum size while it was increased on raising the pH. The post-antimicrobial effect of 100 fg/ml azelastine was also determined and reached up to 3.36 h.Sociedade Brasileira de Microbiologia2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300018Brazilian Journal of Microbiology v.42 n.3 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000300018info:eu-repo/semantics/openAccessEl-Nakeeb,Moustafa AAbou-Shleib,Hamida MKhalil,Amal MOmar,Hoda GEl-Halfawy,Omar Meng2011-12-21T00:00:00Zoai:scielo:S1517-83822011000300018Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-12-21T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
title In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
spellingShingle In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
El-Nakeeb,Moustafa A
Antihistaminics
bactericidal activity
bacteriostatic activity
Gram-negative isolates
Gram-positive isolates
title_short In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
title_full In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
title_fullStr In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
title_full_unstemmed In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
title_sort In vitro antibacterial activity of some antihistaminics belonging to different groups against multi-drug resistant clinical isolates
author El-Nakeeb,Moustafa A
author_facet El-Nakeeb,Moustafa A
Abou-Shleib,Hamida M
Khalil,Amal M
Omar,Hoda G
El-Halfawy,Omar M
author_role author
author2 Abou-Shleib,Hamida M
Khalil,Amal M
Omar,Hoda G
El-Halfawy,Omar M
author2_role author
author
author
author
dc.contributor.author.fl_str_mv El-Nakeeb,Moustafa A
Abou-Shleib,Hamida M
Khalil,Amal M
Omar,Hoda G
El-Halfawy,Omar M
dc.subject.por.fl_str_mv Antihistaminics
bactericidal activity
bacteriostatic activity
Gram-negative isolates
Gram-positive isolates
topic Antihistaminics
bactericidal activity
bacteriostatic activity
Gram-negative isolates
Gram-positive isolates
description Antihistaminics are widely used for various indications during microbial infection. Hence, this paper investigates the antimicrobial activities of 10 antihistaminics belonging to both old and new generations using multiresistant Gram-positive and Gram-negative clinical isolates. The bacteriostatic activity of antihistaminics was investigated by determining their MIC both by broth and agar dilution techniques against 29 bacterial strains. Azelastine, cyproheptadine, mequitazine and promethazine were the most active among the tested drugs. Diphenhydramine and cetirizine possessed weaker activity whereas doxylamine, fexofenadine and loratadine were inactive even at the highest tested concentration (1 mg/ml). The MIC of meclozine could not be determined as it precipitated with the used culture media. The MBC values of antihistaminics were almost identical to the corresponding MIC values. The bactericidal activity of antihistaminics was also studied by the viable count technique in sterile saline solution. Evident killing effects were exerted by mequitazine, meclozine, azelastine and cyproheptadine. Moreover, the dynamics of bactericidal activity of azelastine were studied by the viable count technique in nutrient broth. This activity was found to be concentration-dependant. This effect was reduced on increasing the inoculum size while it was increased on raising the pH. The post-antimicrobial effect of 100 fg/ml azelastine was also determined and reached up to 3.36 h.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822011000300018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.42 n.3 2011
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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