Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification

Detalhes bibliográficos
Autor(a) principal: Lallo da Silva, Bruna [UNESP]
Data de Publicação: 2019
Outros Autores: Caetano, Bruno Leonardo [UNESP], Chiari-Andréo, Bruna Galdorfini [UNESP], Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP], Chiavacci, Leila Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.colsurfb.2019.02.013
http://hdl.handle.net/11449/188747
Resumo: In the current study, the size and surface of ZnO nanoparticle (ZnO NP) suspensions and powders were finely controlled to evaluate their influence on the ZnO antibacterial activity against Staphylococcus aureus and Escherichia coli. The ZnO NP were prepared by the sol-gel method with different reaction times for NP size control and followed by the addition of (3-glycidyloxypropyl) trimethoxysilane (GPTMS) as a surface modifier. The ZnO NP were characterized by different techniques and the antibacterial activity was assessed through the minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC) and scanning electron microscopy (SEM). The ZnO NP exhibited significant antibacterial activity against Staphylococcus aureus. The NP size highly influenced the antibacterial activity, which increased with decreasing particle size. The small ZnO NP presented bactericidal activity whereas the largest showed bacteriostatic activity. The use of GPTMS, in general, led to increase of MIC and MBC. The formation of holes in the cell wall of Staphylococcus aureus was evidenced by SEM after contact between the bacteria and ZnO NP. The cytotoxicity assay showed that ZnO NP did not cause a loss of cell viability in the human keratinocyte cell line (HaCat) at the maximum concentration assessed. Thus, this study indicated that 5 nm ZnO NP modified by GPTMS has great potential for use as an inorganic antibacterial material.
id UNSP_955a590aba8a1eafedb7eccf022a3c97
oai_identifier_str oai:repositorio.unesp.br:11449/188747
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modificationBactericidalBacteriostaticNanoparticlesSizeSurfaceZnOIn the current study, the size and surface of ZnO nanoparticle (ZnO NP) suspensions and powders were finely controlled to evaluate their influence on the ZnO antibacterial activity against Staphylococcus aureus and Escherichia coli. The ZnO NP were prepared by the sol-gel method with different reaction times for NP size control and followed by the addition of (3-glycidyloxypropyl) trimethoxysilane (GPTMS) as a surface modifier. The ZnO NP were characterized by different techniques and the antibacterial activity was assessed through the minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC) and scanning electron microscopy (SEM). The ZnO NP exhibited significant antibacterial activity against Staphylococcus aureus. The NP size highly influenced the antibacterial activity, which increased with decreasing particle size. The small ZnO NP presented bactericidal activity whereas the largest showed bacteriostatic activity. The use of GPTMS, in general, led to increase of MIC and MBC. The formation of holes in the cell wall of Staphylococcus aureus was evidenced by SEM after contact between the bacteria and ZnO NP. The cytotoxicity assay showed that ZnO NP did not cause a loss of cell viability in the human keratinocyte cell line (HaCat) at the maximum concentration assessed. Thus, this study indicated that 5 nm ZnO NP modified by GPTMS has great potential for use as an inorganic antibacterial material.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Highway Araraquara-JaúDepartment of Biological and Health Sciences Universidade de Araraquara UNIARASão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Highway Araraquara-JaúUniversidade Estadual Paulista (Unesp)UNIARALallo da Silva, Bruna [UNESP]Caetano, Bruno Leonardo [UNESP]Chiari-Andréo, Bruna Galdorfini [UNESP]Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP]Chiavacci, Leila Aparecida [UNESP]2019-10-06T16:17:58Z2019-10-06T16:17:58Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article440-447http://dx.doi.org/10.1016/j.colsurfb.2019.02.013Colloids and Surfaces B: Biointerfaces, v. 177, p. 440-447.1873-43670927-7765http://hdl.handle.net/11449/18874710.1016/j.colsurfb.2019.02.0132-s2.0-85061790701Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfacesinfo:eu-repo/semantics/openAccess2021-10-23T19:23:44Zoai:repositorio.unesp.br:11449/188747Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
title Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
spellingShingle Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
Lallo da Silva, Bruna [UNESP]
Bactericidal
Bacteriostatic
Nanoparticles
Size
Surface
ZnO
title_short Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
title_full Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
title_fullStr Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
title_full_unstemmed Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
title_sort Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
author Lallo da Silva, Bruna [UNESP]
author_facet Lallo da Silva, Bruna [UNESP]
Caetano, Bruno Leonardo [UNESP]
Chiari-Andréo, Bruna Galdorfini [UNESP]
Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP]
Chiavacci, Leila Aparecida [UNESP]
author_role author
author2 Caetano, Bruno Leonardo [UNESP]
Chiari-Andréo, Bruna Galdorfini [UNESP]
Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP]
Chiavacci, Leila Aparecida [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNIARA
dc.contributor.author.fl_str_mv Lallo da Silva, Bruna [UNESP]
Caetano, Bruno Leonardo [UNESP]
Chiari-Andréo, Bruna Galdorfini [UNESP]
Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP]
Chiavacci, Leila Aparecida [UNESP]
dc.subject.por.fl_str_mv Bactericidal
Bacteriostatic
Nanoparticles
Size
Surface
ZnO
topic Bactericidal
Bacteriostatic
Nanoparticles
Size
Surface
ZnO
description In the current study, the size and surface of ZnO nanoparticle (ZnO NP) suspensions and powders were finely controlled to evaluate their influence on the ZnO antibacterial activity against Staphylococcus aureus and Escherichia coli. The ZnO NP were prepared by the sol-gel method with different reaction times for NP size control and followed by the addition of (3-glycidyloxypropyl) trimethoxysilane (GPTMS) as a surface modifier. The ZnO NP were characterized by different techniques and the antibacterial activity was assessed through the minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC) and scanning electron microscopy (SEM). The ZnO NP exhibited significant antibacterial activity against Staphylococcus aureus. The NP size highly influenced the antibacterial activity, which increased with decreasing particle size. The small ZnO NP presented bactericidal activity whereas the largest showed bacteriostatic activity. The use of GPTMS, in general, led to increase of MIC and MBC. The formation of holes in the cell wall of Staphylococcus aureus was evidenced by SEM after contact between the bacteria and ZnO NP. The cytotoxicity assay showed that ZnO NP did not cause a loss of cell viability in the human keratinocyte cell line (HaCat) at the maximum concentration assessed. Thus, this study indicated that 5 nm ZnO NP modified by GPTMS has great potential for use as an inorganic antibacterial material.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:17:58Z
2019-10-06T16:17:58Z
2019-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.colsurfb.2019.02.013
Colloids and Surfaces B: Biointerfaces, v. 177, p. 440-447.
1873-4367
0927-7765
http://hdl.handle.net/11449/188747
10.1016/j.colsurfb.2019.02.013
2-s2.0-85061790701
url http://dx.doi.org/10.1016/j.colsurfb.2019.02.013
http://hdl.handle.net/11449/188747
identifier_str_mv Colloids and Surfaces B: Biointerfaces, v. 177, p. 440-447.
1873-4367
0927-7765
10.1016/j.colsurfb.2019.02.013
2-s2.0-85061790701
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Colloids and Surfaces B: Biointerfaces
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 440-447
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965611081596928