Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.colsurfb.2019.02.013 http://hdl.handle.net/11449/188747 |
Resumo: | In the current study, the size and surface of ZnO nanoparticle (ZnO NP) suspensions and powders were finely controlled to evaluate their influence on the ZnO antibacterial activity against Staphylococcus aureus and Escherichia coli. The ZnO NP were prepared by the sol-gel method with different reaction times for NP size control and followed by the addition of (3-glycidyloxypropyl) trimethoxysilane (GPTMS) as a surface modifier. The ZnO NP were characterized by different techniques and the antibacterial activity was assessed through the minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC) and scanning electron microscopy (SEM). The ZnO NP exhibited significant antibacterial activity against Staphylococcus aureus. The NP size highly influenced the antibacterial activity, which increased with decreasing particle size. The small ZnO NP presented bactericidal activity whereas the largest showed bacteriostatic activity. The use of GPTMS, in general, led to increase of MIC and MBC. The formation of holes in the cell wall of Staphylococcus aureus was evidenced by SEM after contact between the bacteria and ZnO NP. The cytotoxicity assay showed that ZnO NP did not cause a loss of cell viability in the human keratinocyte cell line (HaCat) at the maximum concentration assessed. Thus, this study indicated that 5 nm ZnO NP modified by GPTMS has great potential for use as an inorganic antibacterial material. |
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Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modificationBactericidalBacteriostaticNanoparticlesSizeSurfaceZnOIn the current study, the size and surface of ZnO nanoparticle (ZnO NP) suspensions and powders were finely controlled to evaluate their influence on the ZnO antibacterial activity against Staphylococcus aureus and Escherichia coli. The ZnO NP were prepared by the sol-gel method with different reaction times for NP size control and followed by the addition of (3-glycidyloxypropyl) trimethoxysilane (GPTMS) as a surface modifier. The ZnO NP were characterized by different techniques and the antibacterial activity was assessed through the minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC) and scanning electron microscopy (SEM). The ZnO NP exhibited significant antibacterial activity against Staphylococcus aureus. The NP size highly influenced the antibacterial activity, which increased with decreasing particle size. The small ZnO NP presented bactericidal activity whereas the largest showed bacteriostatic activity. The use of GPTMS, in general, led to increase of MIC and MBC. The formation of holes in the cell wall of Staphylococcus aureus was evidenced by SEM after contact between the bacteria and ZnO NP. The cytotoxicity assay showed that ZnO NP did not cause a loss of cell viability in the human keratinocyte cell line (HaCat) at the maximum concentration assessed. Thus, this study indicated that 5 nm ZnO NP modified by GPTMS has great potential for use as an inorganic antibacterial material.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Highway Araraquara-JaúDepartment of Biological and Health Sciences Universidade de Araraquara UNIARASão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, Highway Araraquara-JaúUniversidade Estadual Paulista (Unesp)UNIARALallo da Silva, Bruna [UNESP]Caetano, Bruno Leonardo [UNESP]Chiari-Andréo, Bruna Galdorfini [UNESP]Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP]Chiavacci, Leila Aparecida [UNESP]2019-10-06T16:17:58Z2019-10-06T16:17:58Z2019-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article440-447http://dx.doi.org/10.1016/j.colsurfb.2019.02.013Colloids and Surfaces B: Biointerfaces, v. 177, p. 440-447.1873-43670927-7765http://hdl.handle.net/11449/18874710.1016/j.colsurfb.2019.02.0132-s2.0-85061790701Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengColloids and Surfaces B: Biointerfacesinfo:eu-repo/semantics/openAccess2024-06-24T13:46:32Zoai:repositorio.unesp.br:11449/188747Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:55:55.311819Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification |
title |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification |
spellingShingle |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification Lallo da Silva, Bruna [UNESP] Bactericidal Bacteriostatic Nanoparticles Size Surface ZnO |
title_short |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification |
title_full |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification |
title_fullStr |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification |
title_full_unstemmed |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification |
title_sort |
Increased antibacterial activity of ZnO nanoparticles: Influence of size and surface modification |
author |
Lallo da Silva, Bruna [UNESP] |
author_facet |
Lallo da Silva, Bruna [UNESP] Caetano, Bruno Leonardo [UNESP] Chiari-Andréo, Bruna Galdorfini [UNESP] Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP] Chiavacci, Leila Aparecida [UNESP] |
author_role |
author |
author2 |
Caetano, Bruno Leonardo [UNESP] Chiari-Andréo, Bruna Galdorfini [UNESP] Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP] Chiavacci, Leila Aparecida [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNIARA |
dc.contributor.author.fl_str_mv |
Lallo da Silva, Bruna [UNESP] Caetano, Bruno Leonardo [UNESP] Chiari-Andréo, Bruna Galdorfini [UNESP] Pietro, Rosemeire Cristina Linhari Rodrigues [UNESP] Chiavacci, Leila Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Bactericidal Bacteriostatic Nanoparticles Size Surface ZnO |
topic |
Bactericidal Bacteriostatic Nanoparticles Size Surface ZnO |
description |
In the current study, the size and surface of ZnO nanoparticle (ZnO NP) suspensions and powders were finely controlled to evaluate their influence on the ZnO antibacterial activity against Staphylococcus aureus and Escherichia coli. The ZnO NP were prepared by the sol-gel method with different reaction times for NP size control and followed by the addition of (3-glycidyloxypropyl) trimethoxysilane (GPTMS) as a surface modifier. The ZnO NP were characterized by different techniques and the antibacterial activity was assessed through the minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC) and scanning electron microscopy (SEM). The ZnO NP exhibited significant antibacterial activity against Staphylococcus aureus. The NP size highly influenced the antibacterial activity, which increased with decreasing particle size. The small ZnO NP presented bactericidal activity whereas the largest showed bacteriostatic activity. The use of GPTMS, in general, led to increase of MIC and MBC. The formation of holes in the cell wall of Staphylococcus aureus was evidenced by SEM after contact between the bacteria and ZnO NP. The cytotoxicity assay showed that ZnO NP did not cause a loss of cell viability in the human keratinocyte cell line (HaCat) at the maximum concentration assessed. Thus, this study indicated that 5 nm ZnO NP modified by GPTMS has great potential for use as an inorganic antibacterial material. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:17:58Z 2019-10-06T16:17:58Z 2019-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.colsurfb.2019.02.013 Colloids and Surfaces B: Biointerfaces, v. 177, p. 440-447. 1873-4367 0927-7765 http://hdl.handle.net/11449/188747 10.1016/j.colsurfb.2019.02.013 2-s2.0-85061790701 |
url |
http://dx.doi.org/10.1016/j.colsurfb.2019.02.013 http://hdl.handle.net/11449/188747 |
identifier_str_mv |
Colloids and Surfaces B: Biointerfaces, v. 177, p. 440-447. 1873-4367 0927-7765 10.1016/j.colsurfb.2019.02.013 2-s2.0-85061790701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids and Surfaces B: Biointerfaces |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
440-447 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129474519105536 |