Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100016 |
Resumo: | Recent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the IgM-Measles ELISA IAL, using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC) data. This study was performed in a positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL presented sensitivity higher than 97.1%, specificity and precision of 97%, and agreement kappa (k) index higher than 0.94 (P < 0.05). Moreover, the IgM antibody profile from measles acute phase revealed by the assay was similar to the reference assay. A practical analysis system for checking the quality of new reagent batches was proposed based on the diagnostic features and agreement kappa index. Our findings suggest that measles antigenic reagents can be produced with reliable quality control system, and supplied to public health laboratories for routine serodiagnosis or population surveys. |
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Brazilian Journal of Microbiology |
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Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagentmeaslesIgM-measles reagentreagent quality controlserodiagnosisseroepidemiologyRecent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the IgM-Measles ELISA IAL, using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC) data. This study was performed in a positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL presented sensitivity higher than 97.1%, specificity and precision of 97%, and agreement kappa (k) index higher than 0.94 (P < 0.05). Moreover, the IgM antibody profile from measles acute phase revealed by the assay was similar to the reference assay. A practical analysis system for checking the quality of new reagent batches was proposed based on the diagnostic features and agreement kappa index. Our findings suggest that measles antigenic reagents can be produced with reliable quality control system, and supplied to public health laboratories for routine serodiagnosis or population surveys.Sociedade Brasileira de Microbiologia2001-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100016Brazilian Journal of Microbiology v.32 n.1 2001reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822001000100016info:eu-repo/semantics/openAccessHoshino-Shimizu,SumieVaz-de-Lima,Lourdes Rehder de AndradeOliveira,Maria Isabel dePereira,Carlos AugustoMoura,Anísio deMendonça,Ronaldo Zucatellieng2001-08-02T00:00:00Zoai:scielo:S1517-83822001000100016Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2001-08-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent |
title |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent |
spellingShingle |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent Hoshino-Shimizu,Sumie measles IgM-measles reagent reagent quality control serodiagnosis seroepidemiology |
title_short |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent |
title_full |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent |
title_fullStr |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent |
title_full_unstemmed |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent |
title_sort |
Measles serodiagnosis: production and evaluation of the IgM-measles ELISA IAL reagent |
author |
Hoshino-Shimizu,Sumie |
author_facet |
Hoshino-Shimizu,Sumie Vaz-de-Lima,Lourdes Rehder de Andrade Oliveira,Maria Isabel de Pereira,Carlos Augusto Moura,Anísio de Mendonça,Ronaldo Zucatelli |
author_role |
author |
author2 |
Vaz-de-Lima,Lourdes Rehder de Andrade Oliveira,Maria Isabel de Pereira,Carlos Augusto Moura,Anísio de Mendonça,Ronaldo Zucatelli |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Hoshino-Shimizu,Sumie Vaz-de-Lima,Lourdes Rehder de Andrade Oliveira,Maria Isabel de Pereira,Carlos Augusto Moura,Anísio de Mendonça,Ronaldo Zucatelli |
dc.subject.por.fl_str_mv |
measles IgM-measles reagent reagent quality control serodiagnosis seroepidemiology |
topic |
measles IgM-measles reagent reagent quality control serodiagnosis seroepidemiology |
description |
Recent measles outbreaks in different countries led to an increase of laboratory measles diagnosis. Thus, we developed the IgM-Measles ELISA IAL, using measles virus antigens obtained from cell cultured in microcarriers in order to supply reagent kits to Brazilian public health laboratories. A batch of antigenic reagent was produced and evaluated in the enzyme immunoassay in comparison with clinical diagnosis and with as reference assay (IgM Capture ELISA CDC) data. This study was performed in a positive panel with 70 serum samples from patients with measles, and a negative panel with 132 samples from patients with unrelated diseases and without recent measles or vaccination history. In relation to other diagnostic methods, the IgM ELISA IAL presented sensitivity higher than 97.1%, specificity and precision of 97%, and agreement kappa (k) index higher than 0.94 (P < 0.05). Moreover, the IgM antibody profile from measles acute phase revealed by the assay was similar to the reference assay. A practical analysis system for checking the quality of new reagent batches was proposed based on the diagnostic features and agreement kappa index. Our findings suggest that measles antigenic reagents can be produced with reliable quality control system, and supplied to public health laboratories for routine serodiagnosis or population surveys. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822001000100016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.32 n.1 2001 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122198887235584 |