Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Detalhes bibliográficos
Autor(a) principal: Leal,C.A.G.
Data de Publicação: 2013
Outros Autores: Carvalho-Castro,G.A., Cottorello,A.C., Leite,R.C., Figueiredo,H.C.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038
Resumo: The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
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spelling Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSDclinical samplessensitivityreal-time PCRWSSVWSDThe aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.Sociedade Brasileira de Microbiologia2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038Brazilian Journal of Microbiology v.44 n.3 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013005000054info:eu-repo/semantics/openAccessLeal,C.A.G.Carvalho-Castro,G.A.Cottorello,A.C.Leite,R.C.Figueiredo,H.C.P.eng2014-02-03T00:00:00Zoai:scielo:S1517-83822013000300038Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-02-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
spellingShingle Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
Leal,C.A.G.
clinical samples
sensitivity
real-time PCR
WSSV
WSD
title_short Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_full Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_fullStr Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_full_unstemmed Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_sort Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
author Leal,C.A.G.
author_facet Leal,C.A.G.
Carvalho-Castro,G.A.
Cottorello,A.C.
Leite,R.C.
Figueiredo,H.C.P.
author_role author
author2 Carvalho-Castro,G.A.
Cottorello,A.C.
Leite,R.C.
Figueiredo,H.C.P.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Leal,C.A.G.
Carvalho-Castro,G.A.
Cottorello,A.C.
Leite,R.C.
Figueiredo,H.C.P.
dc.subject.por.fl_str_mv clinical samples
sensitivity
real-time PCR
WSSV
WSD
topic clinical samples
sensitivity
real-time PCR
WSSV
WSD
description The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300038
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822013005000054
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.44 n.3 2013
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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