Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Detalhes bibliográficos
Autor(a) principal: Leal, C. A. G.
Data de Publicação: 2013
Outros Autores: Carvalho-Castro, G. A., Cottorello, A. C., Leite, R. C., Figueiredo, H. C. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/37527
Resumo: The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
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spelling Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSDClinical samplesSensitivityWhite spot syndrome virus (WSSV)Real-time PCRWhite spot disease (WSD)The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.Sociedade Brasileira de Microbiologia2019-11-04T12:53:51Z2019-11-04T12:53:51Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLEAL, C. A. G. et al. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD. Brazilian Journal of Microbiology, São Paulo, v. 44, n. 3, July/Sept. 2013.http://repositorio.ufla.br/jspui/handle/1/37527Brazilian Journal of Microbiologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessLeal, C. A. G.Carvalho-Castro, G. A.Cottorello, A. C.Leite, R. C.Figueiredo, H. C. P.eng2019-11-04T12:54:26Zoai:localhost:1/37527Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-11-04T12:54:26Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
spellingShingle Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
Leal, C. A. G.
Clinical samples
Sensitivity
White spot syndrome virus (WSSV)
Real-time PCR
White spot disease (WSD)
title_short Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_full Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_fullStr Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_full_unstemmed Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
title_sort Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD
author Leal, C. A. G.
author_facet Leal, C. A. G.
Carvalho-Castro, G. A.
Cottorello, A. C.
Leite, R. C.
Figueiredo, H. C. P.
author_role author
author2 Carvalho-Castro, G. A.
Cottorello, A. C.
Leite, R. C.
Figueiredo, H. C. P.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Leal, C. A. G.
Carvalho-Castro, G. A.
Cottorello, A. C.
Leite, R. C.
Figueiredo, H. C. P.
dc.subject.por.fl_str_mv Clinical samples
Sensitivity
White spot syndrome virus (WSSV)
Real-time PCR
White spot disease (WSD)
topic Clinical samples
Sensitivity
White spot syndrome virus (WSSV)
Real-time PCR
White spot disease (WSD)
description The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.
publishDate 2013
dc.date.none.fl_str_mv 2013
2019-11-04T12:53:51Z
2019-11-04T12:53:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LEAL, C. A. G. et al. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD. Brazilian Journal of Microbiology, São Paulo, v. 44, n. 3, July/Sept. 2013.
http://repositorio.ufla.br/jspui/handle/1/37527
identifier_str_mv LEAL, C. A. G. et al. Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD. Brazilian Journal of Microbiology, São Paulo, v. 44, n. 3, July/Sept. 2013.
url http://repositorio.ufla.br/jspui/handle/1/37527
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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