Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk

Detalhes bibliográficos
Autor(a) principal: Martins,Katheryne Benini
Data de Publicação: 2015
Outros Autores: Faccioli-Martins,Patricia Yoshida, Riboli,Danilo Flávio Moraes, Pereira,Valéria Cataneli, Fernandes,Simone, Oliveira,Aline A., Dantas,Ariane, Zafalon,Luiz Francisco, Cunha,Maria de Lourdes Ribeiro de Souza da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535
Resumo: The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.
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spelling Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milkmastitissheepStaphylococcus aureusantibiotic resistancevirulence factorsThe objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.Sociedade Brasileira de Microbiologia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535Brazilian Journal of Microbiology v.46 n.2 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246220131164info:eu-repo/semantics/openAccessMartins,Katheryne BeniniFaccioli-Martins,Patricia YoshidaRiboli,Danilo Flávio MoraesPereira,Valéria CataneliFernandes,SimoneOliveira,Aline A.Dantas,ArianeZafalon,Luiz FranciscoCunha,Maria de Lourdes Ribeiro de Souza daeng2015-11-27T00:00:00Zoai:scielo:S1517-83822015000200535Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-11-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
title Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
spellingShingle Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Martins,Katheryne Benini
mastitis
sheep
Staphylococcus aureus
antibiotic resistance
virulence factors
title_short Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
title_full Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
title_fullStr Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
title_full_unstemmed Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
title_sort Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
author Martins,Katheryne Benini
author_facet Martins,Katheryne Benini
Faccioli-Martins,Patricia Yoshida
Riboli,Danilo Flávio Moraes
Pereira,Valéria Cataneli
Fernandes,Simone
Oliveira,Aline A.
Dantas,Ariane
Zafalon,Luiz Francisco
Cunha,Maria de Lourdes Ribeiro de Souza da
author_role author
author2 Faccioli-Martins,Patricia Yoshida
Riboli,Danilo Flávio Moraes
Pereira,Valéria Cataneli
Fernandes,Simone
Oliveira,Aline A.
Dantas,Ariane
Zafalon,Luiz Francisco
Cunha,Maria de Lourdes Ribeiro de Souza da
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins,Katheryne Benini
Faccioli-Martins,Patricia Yoshida
Riboli,Danilo Flávio Moraes
Pereira,Valéria Cataneli
Fernandes,Simone
Oliveira,Aline A.
Dantas,Ariane
Zafalon,Luiz Francisco
Cunha,Maria de Lourdes Ribeiro de Souza da
dc.subject.por.fl_str_mv mastitis
sheep
Staphylococcus aureus
antibiotic resistance
virulence factors
topic mastitis
sheep
Staphylococcus aureus
antibiotic resistance
virulence factors
description The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246220131164
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.2 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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