Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535 |
Resumo: | The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene. |
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Brazilian Journal of Microbiology |
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Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milkmastitissheepStaphylococcus aureusantibiotic resistancevirulence factorsThe objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.Sociedade Brasileira de Microbiologia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535Brazilian Journal of Microbiology v.46 n.2 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246220131164info:eu-repo/semantics/openAccessMartins,Katheryne BeniniFaccioli-Martins,Patricia YoshidaRiboli,Danilo Flávio MoraesPereira,Valéria CataneliFernandes,SimoneOliveira,Aline A.Dantas,ArianeZafalon,Luiz FranciscoCunha,Maria de Lourdes Ribeiro de Souza daeng2015-11-27T00:00:00Zoai:scielo:S1517-83822015000200535Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-11-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
spellingShingle |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk Martins,Katheryne Benini mastitis sheep Staphylococcus aureus antibiotic resistance virulence factors |
title_short |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_full |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_fullStr |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_full_unstemmed |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_sort |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
author |
Martins,Katheryne Benini |
author_facet |
Martins,Katheryne Benini Faccioli-Martins,Patricia Yoshida Riboli,Danilo Flávio Moraes Pereira,Valéria Cataneli Fernandes,Simone Oliveira,Aline A. Dantas,Ariane Zafalon,Luiz Francisco Cunha,Maria de Lourdes Ribeiro de Souza da |
author_role |
author |
author2 |
Faccioli-Martins,Patricia Yoshida Riboli,Danilo Flávio Moraes Pereira,Valéria Cataneli Fernandes,Simone Oliveira,Aline A. Dantas,Ariane Zafalon,Luiz Francisco Cunha,Maria de Lourdes Ribeiro de Souza da |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Martins,Katheryne Benini Faccioli-Martins,Patricia Yoshida Riboli,Danilo Flávio Moraes Pereira,Valéria Cataneli Fernandes,Simone Oliveira,Aline A. Dantas,Ariane Zafalon,Luiz Francisco Cunha,Maria de Lourdes Ribeiro de Souza da |
dc.subject.por.fl_str_mv |
mastitis sheep Staphylococcus aureus antibiotic resistance virulence factors |
topic |
mastitis sheep Staphylococcus aureus antibiotic resistance virulence factors |
description |
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-838246220131164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.46 n.2 2015 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122207474024448 |