Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/127349 |
Resumo: | The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene. |
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Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milkMastitisSheepStaphylococcus aureusAntibiotic resistanceVirulence factorsThe objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e ImunologiaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Produção AnimalEmbrapa Pecuária SudesteUniversidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e ImunologiaUniversidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Produção AnimalSociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (Embrapa)Martins, Katheryne Benini [UNESP]Faccioli-Martins, Patricia Yoshida [UNESP]Riboli, Danilo Flávio Moraes [UNESP]Pereira, Valéria Cataneli [UNESP]Fernandes, Simone [UNESP]Oliveira, Aline A. [UNESP]Dantas, Ariane [UNESP]Zafalon, Luiz FranciscoCunha, Maria De Lourdes Ribeiro De Souza da [UNESP]2015-08-26T19:19:16Z2015-08-26T19:19:16Z2015-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article535-543application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535&lng=en&nrm=iso&tlng=enBrazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, 2015.1517-8382http://hdl.handle.net/11449/12734910.1590/S1517-838246220131164S1517-83822015000200535S1517-83822015000200535.pdf0115647772315973SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2023-11-05T06:07:33Zoai:repositorio.unesp.br:11449/127349Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-05T06:07:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
spellingShingle |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk Martins, Katheryne Benini [UNESP] Mastitis Sheep Staphylococcus aureus Antibiotic resistance Virulence factors |
title_short |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_full |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_fullStr |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_full_unstemmed |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
title_sort |
Clonal profile, virulence and resistance of Staphylococcus aureus isolated from sheep milk |
author |
Martins, Katheryne Benini [UNESP] |
author_facet |
Martins, Katheryne Benini [UNESP] Faccioli-Martins, Patricia Yoshida [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Pereira, Valéria Cataneli [UNESP] Fernandes, Simone [UNESP] Oliveira, Aline A. [UNESP] Dantas, Ariane [UNESP] Zafalon, Luiz Francisco Cunha, Maria De Lourdes Ribeiro De Souza da [UNESP] |
author_role |
author |
author2 |
Faccioli-Martins, Patricia Yoshida [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Pereira, Valéria Cataneli [UNESP] Fernandes, Simone [UNESP] Oliveira, Aline A. [UNESP] Dantas, Ariane [UNESP] Zafalon, Luiz Francisco Cunha, Maria De Lourdes Ribeiro De Souza da [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
dc.contributor.author.fl_str_mv |
Martins, Katheryne Benini [UNESP] Faccioli-Martins, Patricia Yoshida [UNESP] Riboli, Danilo Flávio Moraes [UNESP] Pereira, Valéria Cataneli [UNESP] Fernandes, Simone [UNESP] Oliveira, Aline A. [UNESP] Dantas, Ariane [UNESP] Zafalon, Luiz Francisco Cunha, Maria De Lourdes Ribeiro De Souza da [UNESP] |
dc.subject.por.fl_str_mv |
Mastitis Sheep Staphylococcus aureus Antibiotic resistance Virulence factors |
topic |
Mastitis Sheep Staphylococcus aureus Antibiotic resistance Virulence factors |
description |
The objective of this study was to characterize the clonal profile, virulence factors and antimicrobial resistance, particularly oxacillin resistance, of Staphylococcus aureus isolated from sheep milk. Milk samples were collected from all teats for the California Mastitis Test (CMT), somatic cell count, identification of S. aureus, investigation in these strains of genes encoding toxins (sea, seb, sec, sed, tst), biofilm (icaA, icaC, icaD, bap), leukocidin (luk-PV) oxacillin resistance by mecA gene detection and susceptibility testing (12 antibiotics). Messenger RNA expression was evaluated by RT-PCR in isolates carrying toxin and biofilm genes. Biofilm formation was also evaluated phenotypically by adherence to polystyrene plates. The clonal profile of S. aureus was investigated by pulsed-field gel electrophoresis. A total of 473 milk samples were collected from 242 animals on three farms and 20 S. aureus strains were isolated and none carried the mecA gene. The two sec gene-positive isolates and the isolates carrying the tst and luk-PV genes were positive by RT-PCR. Staphylococcus aureus isolated from the three flocks studied showed high susceptibility to the drugs tested and none was biofilm producer, indicating that biofilm formation was not a virulence factor causing infection by these strains. The typing of 17 S. aureus isolates revealed the presence of a common clone on the three farms studied, and the presence and expression of the sec and tst genes in one strain of this clone suggest the possible acquisition of virulence genes by this clone, a fact that is important for animal health and food hygiene. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-26T19:19:16Z 2015-08-26T19:19:16Z 2015-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535&lng=en&nrm=iso&tlng=en Brazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, 2015. 1517-8382 http://hdl.handle.net/11449/127349 10.1590/S1517-838246220131164 S1517-83822015000200535 S1517-83822015000200535.pdf 0115647772315973 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200535&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/127349 |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 46, n. 2, p. 535-543, 2015. 1517-8382 10.1590/S1517-838246220131164 S1517-83822015000200535 S1517-83822015000200535.pdf 0115647772315973 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
535-543 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789614277132288 |