Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200030 |
Resumo: | Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study. |
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Brazilian Journal of Microbiology |
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Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodologyClavulanic acidresponse surface methodologyproductionoptimizationstreptomyces spClavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study.Sociedade Brasileira de Microbiologia2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200030Brazilian Journal of Microbiology v.42 n.2 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000200030info:eu-repo/semantics/openAccessMarques,Daniela A. VianaCunha,Márcia N. CarneiroAraújo,Janete M.Lima-Filho,José L.Converti,AttilioPessoa-Jr,AdalbertoPorto,Ana L. Figueiredoeng2011-06-06T00:00:00Zoai:scielo:S1517-83822011000200030Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-06-06T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology |
title |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology |
spellingShingle |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology Marques,Daniela A. Viana Clavulanic acid response surface methodology production optimization streptomyces sp |
title_short |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology |
title_full |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology |
title_fullStr |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology |
title_full_unstemmed |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology |
title_sort |
Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology |
author |
Marques,Daniela A. Viana |
author_facet |
Marques,Daniela A. Viana Cunha,Márcia N. Carneiro Araújo,Janete M. Lima-Filho,José L. Converti,Attilio Pessoa-Jr,Adalberto Porto,Ana L. Figueiredo |
author_role |
author |
author2 |
Cunha,Márcia N. Carneiro Araújo,Janete M. Lima-Filho,José L. Converti,Attilio Pessoa-Jr,Adalberto Porto,Ana L. Figueiredo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Marques,Daniela A. Viana Cunha,Márcia N. Carneiro Araújo,Janete M. Lima-Filho,José L. Converti,Attilio Pessoa-Jr,Adalberto Porto,Ana L. Figueiredo |
dc.subject.por.fl_str_mv |
Clavulanic acid response surface methodology production optimization streptomyces sp |
topic |
Clavulanic acid response surface methodology production optimization streptomyces sp |
description |
Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200030 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200030 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822011000200030 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.42 n.2 2011 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203573321728 |