Vanillin production by recombinant strains of Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Converti,Attilio
Data de Publicação: 2003
Outros Autores: Faveri,Danilo de, Perego,Patrizia, Barghini,Paolo, Ruzzi,Maurizio, Sene,Luciane
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500037
Resumo: Vanillin production from ferulate was studied using different recombinant strains of Escherichia coli. To prevent the occurrence of aerobic conditions and then possible product oxidation, tests were performed in Erlenmeyer flasks under mild mixing (150 rpm). Among other transformants, E. coli JM109(pBB1) appeared to be the best vanillin producer, being able to convert no less than 95% of starting ferulate to the product within 1h. This yield decreased down to 72% after 72h, likely because of a non-specific oxidase activity responsible for vanillin oxidation to vanillate.
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spelling Vanillin production by recombinant strains of Escherichia colivanillinferulateEscherichia coli JM109recombinant strainsVanillin production from ferulate was studied using different recombinant strains of Escherichia coli. To prevent the occurrence of aerobic conditions and then possible product oxidation, tests were performed in Erlenmeyer flasks under mild mixing (150 rpm). Among other transformants, E. coli JM109(pBB1) appeared to be the best vanillin producer, being able to convert no less than 95% of starting ferulate to the product within 1h. This yield decreased down to 72% after 72h, likely because of a non-specific oxidase activity responsible for vanillin oxidation to vanillate.Sociedade Brasileira de Microbiologia2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500037Brazilian Journal of Microbiology v.34 suppl.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000500037info:eu-repo/semantics/openAccessConverti,AttilioFaveri,Danilo dePerego,PatriziaBarghini,PaoloRuzzi,MaurizioSene,Lucianeeng2004-11-30T00:00:00Zoai:scielo:S1517-83822003000500037Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Vanillin production by recombinant strains of Escherichia coli
title Vanillin production by recombinant strains of Escherichia coli
spellingShingle Vanillin production by recombinant strains of Escherichia coli
Converti,Attilio
vanillin
ferulate
Escherichia coli JM109
recombinant strains
title_short Vanillin production by recombinant strains of Escherichia coli
title_full Vanillin production by recombinant strains of Escherichia coli
title_fullStr Vanillin production by recombinant strains of Escherichia coli
title_full_unstemmed Vanillin production by recombinant strains of Escherichia coli
title_sort Vanillin production by recombinant strains of Escherichia coli
author Converti,Attilio
author_facet Converti,Attilio
Faveri,Danilo de
Perego,Patrizia
Barghini,Paolo
Ruzzi,Maurizio
Sene,Luciane
author_role author
author2 Faveri,Danilo de
Perego,Patrizia
Barghini,Paolo
Ruzzi,Maurizio
Sene,Luciane
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Converti,Attilio
Faveri,Danilo de
Perego,Patrizia
Barghini,Paolo
Ruzzi,Maurizio
Sene,Luciane
dc.subject.por.fl_str_mv vanillin
ferulate
Escherichia coli JM109
recombinant strains
topic vanillin
ferulate
Escherichia coli JM109
recombinant strains
description Vanillin production from ferulate was studied using different recombinant strains of Escherichia coli. To prevent the occurrence of aerobic conditions and then possible product oxidation, tests were performed in Erlenmeyer flasks under mild mixing (150 rpm). Among other transformants, E. coli JM109(pBB1) appeared to be the best vanillin producer, being able to convert no less than 95% of starting ferulate to the product within 1h. This yield decreased down to 72% after 72h, likely because of a non-specific oxidase activity responsible for vanillin oxidation to vanillate.
publishDate 2003
dc.date.none.fl_str_mv 2003-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500037
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500037
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000500037
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 suppl.1 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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