Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/32590 |
Resumo: | With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of eIF antigen from Leishmania chagasi in recombinant Escherichia coli. An induction procedure using IPTG was carried out in a series of trials, to observe the influence of culture medium (2xTY, TB) under expression of the recombinant eIF protein. Results showed that recombinant protein expression was associated to growth and that the highest eIF antigen expression was obtained in the 2xTY medium |
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Santos, Everaldo Silvino dosVaz, Michelle Rossana FerreiraFrança, Ricardo Luiz Soares deAndrade, Sirtys Santos Lessa deSousa Junior, Francisco Canindé deMartins, Daniella Regina ArantesMacedo, Gorete Ribeiro de2021-05-28T14:58:00Z2021-05-28T14:58:00Z2011-12VAZ, M. R. F.; FRANCA, R. L. S.; ANDRADE, S. S. L.; SOUSA JUNIOR, F. C.; SANTOS, E. S.; MARTINS, D. R. A.; MACEDO, G. R.. Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli. Brazilian Journal of Microbiology (Impresso), v. 42, p. 1390-1396, 2011. Disponível em: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021&lng=en&nrm=iso&tlng=en. Acesso em: 15 abr. 2021. http://dx.doi.org/10.1590/S1517-83822011000400021.1517-8382https://repositorio.ufrn.br/handle/123456789/3259010.1590/S1517-83822011000400021Sociedade Brasileira de MicrobiologiaAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessCultivationRecombinant proteinVisceral leishmaniasisEscherichia coliInfluence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleWith the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of eIF antigen from Leishmania chagasi in recombinant Escherichia coli. An induction procedure using IPTG was carried out in a series of trials, to observe the influence of culture medium (2xTY, TB) under expression of the recombinant eIF protein. Results showed that recombinant protein expression was associated to growth and that the highest eIF antigen expression was obtained in the 2xTY mediumengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALInfluenceCultureMedium_Santos_2011.pdfInfluenceCultureMedium_Santos_2011.pdfapplication/pdf317641https://repositorio.ufrn.br/bitstream/123456789/32590/1/InfluenceCultureMedium_Santos_2011.pdf6347af0e9e899798a1ea0c3ee32e07efMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.ufrn.br/bitstream/123456789/32590/2/license_rdfe39d27027a6cc9cb039ad269a5db8e34MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32590/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/325902021-05-28 11:58:00.831oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-28T14:58Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli |
title |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli |
spellingShingle |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli Santos, Everaldo Silvino dos Cultivation Recombinant protein Visceral leishmaniasis Escherichia coli |
title_short |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli |
title_full |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli |
title_fullStr |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli |
title_full_unstemmed |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli |
title_sort |
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli |
author |
Santos, Everaldo Silvino dos |
author_facet |
Santos, Everaldo Silvino dos Vaz, Michelle Rossana Ferreira França, Ricardo Luiz Soares de Andrade, Sirtys Santos Lessa de Sousa Junior, Francisco Canindé de Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro de |
author_role |
author |
author2 |
Vaz, Michelle Rossana Ferreira França, Ricardo Luiz Soares de Andrade, Sirtys Santos Lessa de Sousa Junior, Francisco Canindé de Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, Everaldo Silvino dos Vaz, Michelle Rossana Ferreira França, Ricardo Luiz Soares de Andrade, Sirtys Santos Lessa de Sousa Junior, Francisco Canindé de Martins, Daniella Regina Arantes Macedo, Gorete Ribeiro de |
dc.subject.por.fl_str_mv |
Cultivation Recombinant protein Visceral leishmaniasis Escherichia coli |
topic |
Cultivation Recombinant protein Visceral leishmaniasis Escherichia coli |
description |
With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of eIF antigen from Leishmania chagasi in recombinant Escherichia coli. An induction procedure using IPTG was carried out in a series of trials, to observe the influence of culture medium (2xTY, TB) under expression of the recombinant eIF protein. Results showed that recombinant protein expression was associated to growth and that the highest eIF antigen expression was obtained in the 2xTY medium |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-12 |
dc.date.accessioned.fl_str_mv |
2021-05-28T14:58:00Z |
dc.date.available.fl_str_mv |
2021-05-28T14:58:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VAZ, M. R. F.; FRANCA, R. L. S.; ANDRADE, S. S. L.; SOUSA JUNIOR, F. C.; SANTOS, E. S.; MARTINS, D. R. A.; MACEDO, G. R.. Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli. Brazilian Journal of Microbiology (Impresso), v. 42, p. 1390-1396, 2011. Disponível em: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021&lng=en&nrm=iso&tlng=en. Acesso em: 15 abr. 2021. http://dx.doi.org/10.1590/S1517-83822011000400021. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/32590 |
dc.identifier.issn.none.fl_str_mv |
1517-8382 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1517-83822011000400021 |
identifier_str_mv |
VAZ, M. R. F.; FRANCA, R. L. S.; ANDRADE, S. S. L.; SOUSA JUNIOR, F. C.; SANTOS, E. S.; MARTINS, D. R. A.; MACEDO, G. R.. Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli. Brazilian Journal of Microbiology (Impresso), v. 42, p. 1390-1396, 2011. Disponível em: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400021&lng=en&nrm=iso&tlng=en. Acesso em: 15 abr. 2021. http://dx.doi.org/10.1590/S1517-83822011000400021. 1517-8382 10.1590/S1517-83822011000400021 |
url |
https://repositorio.ufrn.br/handle/123456789/32590 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
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openAccess |
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Sociedade Brasileira de Microbiologia |
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Sociedade Brasileira de Microbiologia |
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