Isolation and characterization of arsenic resistant bacteria from wastewater

Detalhes bibliográficos
Autor(a) principal: Abbas,Syed Zaghum
Data de Publicação: 2014
Outros Autores: Riaz,Mehwish, Ramzan,Naseem, Zahid,M. Tariq, Shakoori,Farah R., Rafatullah,Mohd.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400022
Resumo: The present study proposed the isolation of arsenic resistant bacteria from wastewater. Only three bacterial isolates (MNZ1, MNZ4 and MNZ6) were able to grow in high concentrations of arsenic. The minimum inhibitory concentrations of arsenic against MNZ1, MNZ4 and MNZ6 were 300 mg/L, 300 mg/L and 370 mg/L respectively. The isolated strains showed maximum growth at 37 ºC and at 7.0 pH in control but in arsenite stress Luria Bertani broth the bacterial growth is lower than control. All strains were arsenite oxidizing. All strains were biochemically characterized and ribotyping (16S rRNA) was done for the purpose of identification which confirmed that MNZ1 was homologous to Enterobacter sp. while MNZ4 and MNZ6 showed their maximum homology with Klebsiella pneumoniae. The protein profiling of these strains showed in arsenic stressed and non stressed conditions, so no bands of induced proteins appeared in stressed conditions. The bacterial isolates can be exploited for bioremediation of arsenic containing wastes, since they seem to have the potential to oxidize the arsenite (more toxic) into arsenate (less toxic) form.
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spelling Isolation and characterization of arsenic resistant bacteria from wastewaterArsenite oxidizing bacteriaBioremediationEnterobacter sp.Klebsiella pneumoniaeThe present study proposed the isolation of arsenic resistant bacteria from wastewater. Only three bacterial isolates (MNZ1, MNZ4 and MNZ6) were able to grow in high concentrations of arsenic. The minimum inhibitory concentrations of arsenic against MNZ1, MNZ4 and MNZ6 were 300 mg/L, 300 mg/L and 370 mg/L respectively. The isolated strains showed maximum growth at 37 ºC and at 7.0 pH in control but in arsenite stress Luria Bertani broth the bacterial growth is lower than control. All strains were arsenite oxidizing. All strains were biochemically characterized and ribotyping (16S rRNA) was done for the purpose of identification which confirmed that MNZ1 was homologous to Enterobacter sp. while MNZ4 and MNZ6 showed their maximum homology with Klebsiella pneumoniae. The protein profiling of these strains showed in arsenic stressed and non stressed conditions, so no bands of induced proteins appeared in stressed conditions. The bacterial isolates can be exploited for bioremediation of arsenic containing wastes, since they seem to have the potential to oxidize the arsenite (more toxic) into arsenate (less toxic) form.Sociedade Brasileira de Microbiologia2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400022Brazilian Journal of Microbiology v.45 n.4 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000400022info:eu-repo/semantics/openAccessAbbas,Syed ZaghumRiaz,MehwishRamzan,NaseemZahid,M. TariqShakoori,Farah R.Rafatullah,Mohd.eng2015-02-19T00:00:00Zoai:scielo:S1517-83822014000400022Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-02-19T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Isolation and characterization of arsenic resistant bacteria from wastewater
title Isolation and characterization of arsenic resistant bacteria from wastewater
spellingShingle Isolation and characterization of arsenic resistant bacteria from wastewater
Abbas,Syed Zaghum
Arsenite oxidizing bacteria
Bioremediation
Enterobacter sp.
Klebsiella pneumoniae
title_short Isolation and characterization of arsenic resistant bacteria from wastewater
title_full Isolation and characterization of arsenic resistant bacteria from wastewater
title_fullStr Isolation and characterization of arsenic resistant bacteria from wastewater
title_full_unstemmed Isolation and characterization of arsenic resistant bacteria from wastewater
title_sort Isolation and characterization of arsenic resistant bacteria from wastewater
author Abbas,Syed Zaghum
author_facet Abbas,Syed Zaghum
Riaz,Mehwish
Ramzan,Naseem
Zahid,M. Tariq
Shakoori,Farah R.
Rafatullah,Mohd.
author_role author
author2 Riaz,Mehwish
Ramzan,Naseem
Zahid,M. Tariq
Shakoori,Farah R.
Rafatullah,Mohd.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Abbas,Syed Zaghum
Riaz,Mehwish
Ramzan,Naseem
Zahid,M. Tariq
Shakoori,Farah R.
Rafatullah,Mohd.
dc.subject.por.fl_str_mv Arsenite oxidizing bacteria
Bioremediation
Enterobacter sp.
Klebsiella pneumoniae
topic Arsenite oxidizing bacteria
Bioremediation
Enterobacter sp.
Klebsiella pneumoniae
description The present study proposed the isolation of arsenic resistant bacteria from wastewater. Only three bacterial isolates (MNZ1, MNZ4 and MNZ6) were able to grow in high concentrations of arsenic. The minimum inhibitory concentrations of arsenic against MNZ1, MNZ4 and MNZ6 were 300 mg/L, 300 mg/L and 370 mg/L respectively. The isolated strains showed maximum growth at 37 ºC and at 7.0 pH in control but in arsenite stress Luria Bertani broth the bacterial growth is lower than control. All strains were arsenite oxidizing. All strains were biochemically characterized and ribotyping (16S rRNA) was done for the purpose of identification which confirmed that MNZ1 was homologous to Enterobacter sp. while MNZ4 and MNZ6 showed their maximum homology with Klebsiella pneumoniae. The protein profiling of these strains showed in arsenic stressed and non stressed conditions, so no bands of induced proteins appeared in stressed conditions. The bacterial isolates can be exploited for bioremediation of arsenic containing wastes, since they seem to have the potential to oxidize the arsenite (more toxic) into arsenate (less toxic) form.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000400022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.4 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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