Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels

Detalhes bibliográficos
Autor(a) principal: Phutela,Urmila
Data de Publicação: 2005
Outros Autores: Dhuna,Vikram, Sandhu,Shobhna, Chadha,B.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100013
Resumo: A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)2SO4 after 2-3 days of incubation at a temperature of 50ºC. Highest enzyme activities of 1116 Ug-1 for pectinase and 1270 Ug-1 for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.
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spelling Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peelsthermophilic fungipectinasepolygalacturonaseA thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)2SO4 after 2-3 days of incubation at a temperature of 50ºC. Highest enzyme activities of 1116 Ug-1 for pectinase and 1270 Ug-1 for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.Sociedade Brasileira de Microbiologia2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100013Brazilian Journal of Microbiology v.36 n.1 2005reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822005000100013info:eu-repo/semantics/openAccessPhutela,UrmilaDhuna,VikramSandhu,ShobhnaChadha,B.S.eng2005-09-12T00:00:00Zoai:scielo:S1517-83822005000100013Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2005-09-12T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
title Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
spellingShingle Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
Phutela,Urmila
thermophilic fungi
pectinase
polygalacturonase
title_short Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
title_full Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
title_fullStr Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
title_full_unstemmed Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
title_sort Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels
author Phutela,Urmila
author_facet Phutela,Urmila
Dhuna,Vikram
Sandhu,Shobhna
Chadha,B.S.
author_role author
author2 Dhuna,Vikram
Sandhu,Shobhna
Chadha,B.S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Phutela,Urmila
Dhuna,Vikram
Sandhu,Shobhna
Chadha,B.S.
dc.subject.por.fl_str_mv thermophilic fungi
pectinase
polygalacturonase
topic thermophilic fungi
pectinase
polygalacturonase
description A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)2SO4 after 2-3 days of incubation at a temperature of 50ºC. Highest enzyme activities of 1116 Ug-1 for pectinase and 1270 Ug-1 for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.
publishDate 2005
dc.date.none.fl_str_mv 2005-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822005000100013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.36 n.1 2005
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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