Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Archives of Biology and Technology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500004 |
Resumo: | A liquid medium containing wheat bran, salts and a source of inducer (pectin) was found to be suitable for the production of exo- and endo-polygalacturonases by Aspergillus oryzae CCT3940. Induction of polygalacturonases by purified pectin was significantly higher than when rinds of citrus fruits were used as inducer. A. oryzae growth was favoured by pH close to 4, although a drop of pH to around 3 was needed for enzymes production. Afterwards, decreasing activities were observed with the normal increase in pH to near neutrality. The highest activities were achieved with an initial pH of 4 and controlled when it decreased to a value slightly below 3 (159 units endo-PG.mL-1 at 83 h and 45 units exo-PG.mL-1 at 64 h), being the loss in polygalacturonases activities strongly reduced at this condition. The best values of pH and temperature for the action of exo-PG (4.5/57ºC) and endo-PG (4.3/40ºC) were assessed. |
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Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzaePectinasepolygalacturonasesubmerged processproduction mediumpHA liquid medium containing wheat bran, salts and a source of inducer (pectin) was found to be suitable for the production of exo- and endo-polygalacturonases by Aspergillus oryzae CCT3940. Induction of polygalacturonases by purified pectin was significantly higher than when rinds of citrus fruits were used as inducer. A. oryzae growth was favoured by pH close to 4, although a drop of pH to around 3 was needed for enzymes production. Afterwards, decreasing activities were observed with the normal increase in pH to near neutrality. The highest activities were achieved with an initial pH of 4 and controlled when it decreased to a value slightly below 3 (159 units endo-PG.mL-1 at 83 h and 45 units exo-PG.mL-1 at 64 h), being the loss in polygalacturonases activities strongly reduced at this condition. The best values of pH and temperature for the action of exo-PG (4.5/57ºC) and endo-PG (4.3/40ºC) were assessed.Instituto de Tecnologia do Paraná - Tecpar2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500004Brazilian Archives of Biology and Technology v.47 n.5 2004reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132004000500004info:eu-repo/semantics/openAccessMalvessi,EloaneSilveira,Mauricio Moura daeng2004-11-23T00:00:00Zoai:scielo:S1516-89132004000500004Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2004-11-23T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false |
dc.title.none.fl_str_mv |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae |
title |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae |
spellingShingle |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae Malvessi,Eloane Pectinase polygalacturonase submerged process production medium pH |
title_short |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae |
title_full |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae |
title_fullStr |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae |
title_full_unstemmed |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae |
title_sort |
Influence of medium composition and pH on the production of polygalacturonases by Aspergillus oryzae |
author |
Malvessi,Eloane |
author_facet |
Malvessi,Eloane Silveira,Mauricio Moura da |
author_role |
author |
author2 |
Silveira,Mauricio Moura da |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Malvessi,Eloane Silveira,Mauricio Moura da |
dc.subject.por.fl_str_mv |
Pectinase polygalacturonase submerged process production medium pH |
topic |
Pectinase polygalacturonase submerged process production medium pH |
description |
A liquid medium containing wheat bran, salts and a source of inducer (pectin) was found to be suitable for the production of exo- and endo-polygalacturonases by Aspergillus oryzae CCT3940. Induction of polygalacturonases by purified pectin was significantly higher than when rinds of citrus fruits were used as inducer. A. oryzae growth was favoured by pH close to 4, although a drop of pH to around 3 was needed for enzymes production. Afterwards, decreasing activities were observed with the normal increase in pH to near neutrality. The highest activities were achieved with an initial pH of 4 and controlled when it decreased to a value slightly below 3 (159 units endo-PG.mL-1 at 83 h and 45 units exo-PG.mL-1 at 64 h), being the loss in polygalacturonases activities strongly reduced at this condition. The best values of pH and temperature for the action of exo-PG (4.5/57ºC) and endo-PG (4.3/40ºC) were assessed. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-89132004000500004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
publisher.none.fl_str_mv |
Instituto de Tecnologia do Paraná - Tecpar |
dc.source.none.fl_str_mv |
Brazilian Archives of Biology and Technology v.47 n.5 2004 reponame:Brazilian Archives of Biology and Technology instname:Instituto de Tecnologia do Paraná (Tecpar) instacron:TECPAR |
instname_str |
Instituto de Tecnologia do Paraná (Tecpar) |
instacron_str |
TECPAR |
institution |
TECPAR |
reponame_str |
Brazilian Archives of Biology and Technology |
collection |
Brazilian Archives of Biology and Technology |
repository.name.fl_str_mv |
Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar) |
repository.mail.fl_str_mv |
babt@tecpar.br||babt@tecpar.br |
_version_ |
1750318269896589312 |