Principles and applications of polymerase chain reaction in medical diagnostic fields: a review
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001 |
Resumo: | Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate platform. The aim of the present literature review is to explore the clinical usefulness and potential of both conventional PCR and Real-Time PCR assays in diverse medical fields, addressing its main uses and advances. |
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Brazilian Journal of Microbiology |
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Principles and applications of polymerase chain reaction in medical diagnostic fields: a reviewConventional PCRReal-Time PCRMolecular BiologyMethods of molecular detectionRecent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate platform. The aim of the present literature review is to explore the clinical usefulness and potential of both conventional PCR and Real-Time PCR assays in diverse medical fields, addressing its main uses and advances.Sociedade Brasileira de Microbiologia2009-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001Brazilian Journal of Microbiology v.40 n.1 2009reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822009000100001info:eu-repo/semantics/openAccessValones,Marcela Agne AlvesGuimarães,Rafael LimaBrandão,Lucas André CavalcantiSouza,Paulo Roberto Eleutério deCarvalho,Alessandra de Albuquerque TavaresCrovela,Sergioeng2009-05-12T00:00:00Zoai:scielo:S1517-83822009000100001Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-05-12T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review |
title |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review |
spellingShingle |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review Valones,Marcela Agne Alves Conventional PCR Real-Time PCR Molecular Biology Methods of molecular detection |
title_short |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review |
title_full |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review |
title_fullStr |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review |
title_full_unstemmed |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review |
title_sort |
Principles and applications of polymerase chain reaction in medical diagnostic fields: a review |
author |
Valones,Marcela Agne Alves |
author_facet |
Valones,Marcela Agne Alves Guimarães,Rafael Lima Brandão,Lucas André Cavalcanti Souza,Paulo Roberto Eleutério de Carvalho,Alessandra de Albuquerque Tavares Crovela,Sergio |
author_role |
author |
author2 |
Guimarães,Rafael Lima Brandão,Lucas André Cavalcanti Souza,Paulo Roberto Eleutério de Carvalho,Alessandra de Albuquerque Tavares Crovela,Sergio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Valones,Marcela Agne Alves Guimarães,Rafael Lima Brandão,Lucas André Cavalcanti Souza,Paulo Roberto Eleutério de Carvalho,Alessandra de Albuquerque Tavares Crovela,Sergio |
dc.subject.por.fl_str_mv |
Conventional PCR Real-Time PCR Molecular Biology Methods of molecular detection |
topic |
Conventional PCR Real-Time PCR Molecular Biology Methods of molecular detection |
description |
Recent developments in molecular methods have revolutionized the detection and characterization of microorganisms in a broad range of medical diagnostic fields, including virology, mycology, parasitology, microbiology and dentistry. Among these methods, Polymerase Chain Reaction (PCR) has generated great benefits and allowed scientific advancements. PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both qualitative and quantitative results, Real-Time PCR is considered a fast and accurate platform. The aim of the present literature review is to explore the clinical usefulness and potential of both conventional PCR and Real-Time PCR assays in diverse medical fields, addressing its main uses and advances. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822009000100001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.40 n.1 2009 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122202195492864 |