Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Detalhes bibliográficos
Autor(a) principal: Miraglia,Fabiana
Data de Publicação: 2008
Outros Autores: Moreno,Andréa Mike, Gomes,Cleise Ribeiro, Paixão,Renata, Liuson,Esequiel, Morais,Zenaide Maria, Maiorka,Paulo, Seixas,Fabiana Kömmling, Dellagostin,Odir Antonio, Vasconcellos,Silvio Arruda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017
Resumo: With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.
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spelling Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, BrazilPomonaSwineVNTRCulturePCRGenital TractWith the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300017info:eu-repo/semantics/openAccessMiraglia,FabianaMoreno,Andréa MikeGomes,Cleise RibeiroPaixão,RenataLiuson,EsequielMorais,Zenaide MariaMaiorka,PauloSeixas,Fabiana KömmlingDellagostin,Odir AntonioVasconcellos,Silvio Arrudaeng2008-10-03T00:00:00Zoai:scielo:S1517-83822008000300017Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
title Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
spellingShingle Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
Miraglia,Fabiana
Pomona
Swine
VNTR
Culture
PCR
Genital Tract
title_short Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
title_full Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
title_fullStr Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
title_full_unstemmed Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
title_sort Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
author Miraglia,Fabiana
author_facet Miraglia,Fabiana
Moreno,Andréa Mike
Gomes,Cleise Ribeiro
Paixão,Renata
Liuson,Esequiel
Morais,Zenaide Maria
Maiorka,Paulo
Seixas,Fabiana Kömmling
Dellagostin,Odir Antonio
Vasconcellos,Silvio Arruda
author_role author
author2 Moreno,Andréa Mike
Gomes,Cleise Ribeiro
Paixão,Renata
Liuson,Esequiel
Morais,Zenaide Maria
Maiorka,Paulo
Seixas,Fabiana Kömmling
Dellagostin,Odir Antonio
Vasconcellos,Silvio Arruda
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Miraglia,Fabiana
Moreno,Andréa Mike
Gomes,Cleise Ribeiro
Paixão,Renata
Liuson,Esequiel
Morais,Zenaide Maria
Maiorka,Paulo
Seixas,Fabiana Kömmling
Dellagostin,Odir Antonio
Vasconcellos,Silvio Arruda
dc.subject.por.fl_str_mv Pomona
Swine
VNTR
Culture
PCR
Genital Tract
topic Pomona
Swine
VNTR
Culture
PCR
Genital Tract
description With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.
publishDate 2008
dc.date.none.fl_str_mv 2008-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822008000300017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.39 n.3 2008
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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