Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017 |
Resumo: | With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona. |
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Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, BrazilPomonaSwineVNTRCulturePCRGenital TractWith the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300017info:eu-repo/semantics/openAccessMiraglia,FabianaMoreno,Andréa MikeGomes,Cleise RibeiroPaixão,RenataLiuson,EsequielMorais,Zenaide MariaMaiorka,PauloSeixas,Fabiana KömmlingDellagostin,Odir AntonioVasconcellos,Silvio Arrudaeng2008-10-03T00:00:00Zoai:scielo:S1517-83822008000300017Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil |
title |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil |
spellingShingle |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil Miraglia,Fabiana Pomona Swine VNTR Culture PCR Genital Tract |
title_short |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil |
title_full |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil |
title_fullStr |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil |
title_full_unstemmed |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil |
title_sort |
Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil |
author |
Miraglia,Fabiana |
author_facet |
Miraglia,Fabiana Moreno,Andréa Mike Gomes,Cleise Ribeiro Paixão,Renata Liuson,Esequiel Morais,Zenaide Maria Maiorka,Paulo Seixas,Fabiana Kömmling Dellagostin,Odir Antonio Vasconcellos,Silvio Arruda |
author_role |
author |
author2 |
Moreno,Andréa Mike Gomes,Cleise Ribeiro Paixão,Renata Liuson,Esequiel Morais,Zenaide Maria Maiorka,Paulo Seixas,Fabiana Kömmling Dellagostin,Odir Antonio Vasconcellos,Silvio Arruda |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Miraglia,Fabiana Moreno,Andréa Mike Gomes,Cleise Ribeiro Paixão,Renata Liuson,Esequiel Morais,Zenaide Maria Maiorka,Paulo Seixas,Fabiana Kömmling Dellagostin,Odir Antonio Vasconcellos,Silvio Arruda |
dc.subject.por.fl_str_mv |
Pomona Swine VNTR Culture PCR Genital Tract |
topic |
Pomona Swine VNTR Culture PCR Genital Tract |
description |
With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000300017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.3 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201824296960 |