Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats

Detalhes bibliográficos
Autor(a) principal: Han,Xufeng
Data de Publicação: 2015
Outros Autores: Wang,Lei, Li,Wei, Li,Bibo, Yang,Yuxin, Yan,Hailong, Qu,Lei, Chen,Yulin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849
Resumo: The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.
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spelling Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goatsgreen fluorescent proteingastro-intestinal tractLactobacillus plantarumgoatThe experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.Sociedade Brasileira de Microbiologia2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849Brazilian Journal of Microbiology v.46 n.3 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246320140556info:eu-repo/semantics/openAccessHan,XufengWang,LeiLi,WeiLi,BiboYang,YuxinYan,HailongQu,LeiChen,Yulineng2015-08-31T00:00:00Zoai:scielo:S1517-83822015000300849Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-08-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
title Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
spellingShingle Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
Han,Xufeng
green fluorescent protein
gastro-intestinal tract
Lactobacillus plantarum
goat
title_short Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
title_full Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
title_fullStr Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
title_full_unstemmed Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
title_sort Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
author Han,Xufeng
author_facet Han,Xufeng
Wang,Lei
Li,Wei
Li,Bibo
Yang,Yuxin
Yan,Hailong
Qu,Lei
Chen,Yulin
author_role author
author2 Wang,Lei
Li,Wei
Li,Bibo
Yang,Yuxin
Yan,Hailong
Qu,Lei
Chen,Yulin
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Han,Xufeng
Wang,Lei
Li,Wei
Li,Bibo
Yang,Yuxin
Yan,Hailong
Qu,Lei
Chen,Yulin
dc.subject.por.fl_str_mv green fluorescent protein
gastro-intestinal tract
Lactobacillus plantarum
goat
topic green fluorescent protein
gastro-intestinal tract
Lactobacillus plantarum
goat
description The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246320140556
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.3 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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