Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849 |
Resumo: | The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum. |
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Brazilian Journal of Microbiology |
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Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goatsgreen fluorescent proteingastro-intestinal tractLactobacillus plantarumgoatThe experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum.Sociedade Brasileira de Microbiologia2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849Brazilian Journal of Microbiology v.46 n.3 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246320140556info:eu-repo/semantics/openAccessHan,XufengWang,LeiLi,WeiLi,BiboYang,YuxinYan,HailongQu,LeiChen,Yulineng2015-08-31T00:00:00Zoai:scielo:S1517-83822015000300849Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-08-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats |
title |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats |
spellingShingle |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats Han,Xufeng green fluorescent protein gastro-intestinal tract Lactobacillus plantarum goat |
title_short |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats |
title_full |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats |
title_fullStr |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats |
title_full_unstemmed |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats |
title_sort |
Use of green fluorescent protein to monitor Lactobacillus plantarum in the gastrointestinal tract of goats |
author |
Han,Xufeng |
author_facet |
Han,Xufeng Wang,Lei Li,Wei Li,Bibo Yang,Yuxin Yan,Hailong Qu,Lei Chen,Yulin |
author_role |
author |
author2 |
Wang,Lei Li,Wei Li,Bibo Yang,Yuxin Yan,Hailong Qu,Lei Chen,Yulin |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Han,Xufeng Wang,Lei Li,Wei Li,Bibo Yang,Yuxin Yan,Hailong Qu,Lei Chen,Yulin |
dc.subject.por.fl_str_mv |
green fluorescent protein gastro-intestinal tract Lactobacillus plantarum goat |
topic |
green fluorescent protein gastro-intestinal tract Lactobacillus plantarum goat |
description |
The experiment aimed to specifically monitor the passage of lactobacilli in vivo after oral administration. The green fluorescent protein (GFP) gene was cloned downstream from the constitutive p32 promoter from L. lactis subsp. cremoris Wg2. The recombinant expression vector, pLEM415-gfp-p32, was electroporated into Lactobacillus plantarum (L. plantarum) isolated from goat. Green fluorescent protein (GFP) was successfully expressed in L. plantarum. After 2 h post-administration, transformed Lactobacillus could be detectable in all luminal contents. In the rumen, bacteria concentration initially decreased, reached the minimum at 42 h post-oral administration and then increased. However, this concentration decreased constantly in the duodenum. This result indicated that L. plantarum could colonize in the rumen but not in the duodenum. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300849 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-838246320140556 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.46 n.3 2015 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122207813763072 |