A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

Detalhes bibliográficos
Autor(a) principal: Angelini,Leticia Mara Lima
Data de Publicação: 2015
Outros Autores: Silva,Amanda Ribeiro Martins da, Rocco,Lucas de Freitas Coli, Milagre,Cintia Duarte de Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113
Resumo: A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
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spelling A high-throughput screening assay for distinguishing nitrile hydratases from nitrilasesnitrile hydrataseamidasenitrilaseamidase inhibitorhigh-throughput screeningA modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.Sociedade Brasileira de Microbiologia2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113Brazilian Journal of Microbiology v.46 n.1 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246120130851info:eu-repo/semantics/openAccessAngelini,Leticia Mara LimaSilva,Amanda Ribeiro Martins daRocco,Lucas de Freitas ColiMilagre,Cintia Duarte de Freitaseng2015-10-27T00:00:00Zoai:scielo:S1517-83822015000100113Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-10-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
spellingShingle A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Angelini,Leticia Mara Lima
nitrile hydratase
amidase
nitrilase
amidase inhibitor
high-throughput screening
title_short A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_full A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_fullStr A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_full_unstemmed A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_sort A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
author Angelini,Leticia Mara Lima
author_facet Angelini,Leticia Mara Lima
Silva,Amanda Ribeiro Martins da
Rocco,Lucas de Freitas Coli
Milagre,Cintia Duarte de Freitas
author_role author
author2 Silva,Amanda Ribeiro Martins da
Rocco,Lucas de Freitas Coli
Milagre,Cintia Duarte de Freitas
author2_role author
author
author
dc.contributor.author.fl_str_mv Angelini,Leticia Mara Lima
Silva,Amanda Ribeiro Martins da
Rocco,Lucas de Freitas Coli
Milagre,Cintia Duarte de Freitas
dc.subject.por.fl_str_mv nitrile hydratase
amidase
nitrilase
amidase inhibitor
high-throughput screening
topic nitrile hydratase
amidase
nitrilase
amidase inhibitor
high-throughput screening
description A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246120130851
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.1 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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