A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

Detalhes bibliográficos
Autor(a) principal: Angelini, Leticia Mara Lima [UNESP]
Data de Publicação: 2015
Outros Autores: Silva, Amanda Ribeiro Martins da [UNESP], Rocco, Lucas de Freitas Coli [UNESP], Milagre, Cintia Duarte de Freitas [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/128702
Resumo: A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
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spelling A high-throughput screening assay for distinguishing nitrile hydratases from nitrilasesNitrile hydrataseAmidaseNitrilaseAmidase inhibitorHigh-throughput screeningA modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista, Departamento de Biologia, Instituto de Biociências de Rio ClaroUniversidade Estadual Paulista, Departamento de Química Orgânica, Instituto de Química de AraraquaraSoc Brasileira MicrobiologiaUniversidade Estadual Paulista (Unesp)Angelini, Leticia Mara Lima [UNESP]Silva, Amanda Ribeiro Martins da [UNESP]Rocco, Lucas de Freitas Coli [UNESP]Milagre, Cintia Duarte de Freitas [UNESP]2015-10-21T13:12:28Z2015-10-21T13:12:28Z2015-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article113-116application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113&lng=en&nrm=iso&tlng=enBrazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.1517-8382http://hdl.handle.net/11449/12870210.1590/S1517-838246120130851S1517-83822015000100113WOS:000355769900013S1517-83822015000100113.pdf14257489168493760000-0001-5627-8616Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-01-11T06:30:12Zoai:repositorio.unesp.br:11449/128702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-11T06:30:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
spellingShingle A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
Angelini, Leticia Mara Lima [UNESP]
Nitrile hydratase
Amidase
Nitrilase
Amidase inhibitor
High-throughput screening
title_short A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_full A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_fullStr A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_full_unstemmed A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
title_sort A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases
author Angelini, Leticia Mara Lima [UNESP]
author_facet Angelini, Leticia Mara Lima [UNESP]
Silva, Amanda Ribeiro Martins da [UNESP]
Rocco, Lucas de Freitas Coli [UNESP]
Milagre, Cintia Duarte de Freitas [UNESP]
author_role author
author2 Silva, Amanda Ribeiro Martins da [UNESP]
Rocco, Lucas de Freitas Coli [UNESP]
Milagre, Cintia Duarte de Freitas [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Angelini, Leticia Mara Lima [UNESP]
Silva, Amanda Ribeiro Martins da [UNESP]
Rocco, Lucas de Freitas Coli [UNESP]
Milagre, Cintia Duarte de Freitas [UNESP]
dc.subject.por.fl_str_mv Nitrile hydratase
Amidase
Nitrilase
Amidase inhibitor
High-throughput screening
topic Nitrile hydratase
Amidase
Nitrilase
Amidase inhibitor
High-throughput screening
description A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T13:12:28Z
2015-10-21T13:12:28Z
2015-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113&lng=en&nrm=iso&tlng=en
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.
1517-8382
http://hdl.handle.net/11449/128702
10.1590/S1517-838246120130851
S1517-83822015000100113
WOS:000355769900013
S1517-83822015000100113.pdf
1425748916849376
0000-0001-5627-8616
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100113&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/128702
identifier_str_mv Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 46, n. 1, p. 113-116, 2015.
1517-8382
10.1590/S1517-838246120130851
S1517-83822015000100113
WOS:000355769900013
S1517-83822015000100113.pdf
1425748916849376
0000-0001-5627-8616
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal Of Microbiology
1.810
0,630
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 113-116
application/pdf
dc.publisher.none.fl_str_mv Soc Brasileira Microbiologia
publisher.none.fl_str_mv Soc Brasileira Microbiologia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965588025507840

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