Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300030 |
Resumo: | Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm. |
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Brazilian Journal of Microbiology |
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spelling |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation processAureobasidium pullulansPullulanNon-stirred fed-batch fermentationTotal 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.Sociedade Brasileira de Microbiologia2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300030Brazilian Journal of Microbiology v.43 n.3 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000300030info:eu-repo/semantics/openAccessSingh,RanjanGaur,RajeevaTiwari,SoniGaur,Manogya Kumareng2012-11-26T00:00:00Zoai:scielo:S1517-83822012000300030Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-11-26T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process |
title |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process |
spellingShingle |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process Singh,Ranjan Aureobasidium pullulans Pullulan Non-stirred fed-batch fermentation |
title_short |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process |
title_full |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process |
title_fullStr |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process |
title_full_unstemmed |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process |
title_sort |
Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process |
author |
Singh,Ranjan |
author_facet |
Singh,Ranjan Gaur,Rajeeva Tiwari,Soni Gaur,Manogya Kumar |
author_role |
author |
author2 |
Gaur,Rajeeva Tiwari,Soni Gaur,Manogya Kumar |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Singh,Ranjan Gaur,Rajeeva Tiwari,Soni Gaur,Manogya Kumar |
dc.subject.por.fl_str_mv |
Aureobasidium pullulans Pullulan Non-stirred fed-batch fermentation |
topic |
Aureobasidium pullulans Pullulan Non-stirred fed-batch fermentation |
description |
Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300030 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300030 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822012000300030 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.43 n.3 2012 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122204721512448 |