Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation

Detalhes bibliográficos
Autor(a) principal: Sotero-Martins,Adriana
Data de Publicação: 2003
Outros Autores: Bon,Elba Pinto da Silva, Carvajal,Elvira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017
Resumo: Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen starvation in vivo.
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spelling Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvationasparaginase IInitrogen regulationSaccharomyces cerevisiaegenomic integrationgreen fluorescent proteinProduction of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen starvation in vivo.Sociedade Brasileira de Microbiologia2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017Brazilian Journal of Microbiology v.34 n.4 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000400017info:eu-repo/semantics/openAccessSotero-Martins,AdrianaBon,Elba Pinto da SilvaCarvajal,Elviraeng2004-08-13T00:00:00Zoai:scielo:S1517-83822003000400017Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-08-13T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
title Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
spellingShingle Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
Sotero-Martins,Adriana
asparaginase II
nitrogen regulation
Saccharomyces cerevisiae
genomic integration
green fluorescent protein
title_short Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
title_full Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
title_fullStr Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
title_full_unstemmed Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
title_sort Asparaginase II-GFP fusion as a tool for studying the secretion of the enzyme under nitrogen starvation
author Sotero-Martins,Adriana
author_facet Sotero-Martins,Adriana
Bon,Elba Pinto da Silva
Carvajal,Elvira
author_role author
author2 Bon,Elba Pinto da Silva
Carvajal,Elvira
author2_role author
author
dc.contributor.author.fl_str_mv Sotero-Martins,Adriana
Bon,Elba Pinto da Silva
Carvajal,Elvira
dc.subject.por.fl_str_mv asparaginase II
nitrogen regulation
Saccharomyces cerevisiae
genomic integration
green fluorescent protein
topic asparaginase II
nitrogen regulation
Saccharomyces cerevisiae
genomic integration
green fluorescent protein
description Production of asparaginase II of Saccharomyces cerevisiae is regulated by nitrogen and can be used as a model system for studying other secreted proteins in yeast. Green fluorescent protein (GFP) from Aequorea victoria was fused to the carboxy-terminus of the enzyme by genomic integration to the locus ASP3 of S. cerevisiae. We determined asparaginase II activity, mRNA ASP3, mRNA ASP3-GFP and GFP fluorescence. Nitrogen starvation in cells carrying the chimera ASP3-GFP caused an increase in fluorescence and in the expression of ASP3. We have shown that cells producing the chimera Asp3-GFPp displayed the same response to nitrogen starvation as control cells. We demonstrated that Asp3-GFPp can be used for studying asparaginase II secretion under nitrogen starvation in vivo.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000400017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 n.4 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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