Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268 |
Resumo: | Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive. |
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Brazilian Journal of Microbiology |
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Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistanceFermentationStressesYeastsMicrosatelliteContaminantsAbstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive.Sociedade Brasileira de Microbiologia2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268Brazilian Journal of Microbiology v.48 n.2 2017reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.09.017info:eu-repo/semantics/openAccessReis,Vanda RenataAntonangelo,Ana Teresa Burlamaqui FaracoBassi,Ana Paula GuarnieriColombi,DéboraCeccato-Antonini,Sandra Reginaeng2017-05-11T00:00:00Zoai:scielo:S1517-83822017000200268Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2017-05-11T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance |
title |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance |
spellingShingle |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance Reis,Vanda Renata Fermentation Stresses Yeasts Microsatellite Contaminants |
title_short |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance |
title_full |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance |
title_fullStr |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance |
title_full_unstemmed |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance |
title_sort |
Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance |
author |
Reis,Vanda Renata |
author_facet |
Reis,Vanda Renata Antonangelo,Ana Teresa Burlamaqui Faraco Bassi,Ana Paula Guarnieri Colombi,Débora Ceccato-Antonini,Sandra Regina |
author_role |
author |
author2 |
Antonangelo,Ana Teresa Burlamaqui Faraco Bassi,Ana Paula Guarnieri Colombi,Débora Ceccato-Antonini,Sandra Regina |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Reis,Vanda Renata Antonangelo,Ana Teresa Burlamaqui Faraco Bassi,Ana Paula Guarnieri Colombi,Débora Ceccato-Antonini,Sandra Regina |
dc.subject.por.fl_str_mv |
Fermentation Stresses Yeasts Microsatellite Contaminants |
topic |
Fermentation Stresses Yeasts Microsatellite Contaminants |
description |
Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cerevisiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.09.017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.48 n.2 2017 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122208885407744 |