Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjm.2016.09.017 http://hdl.handle.net/11449/162799 |
Resumo: | Strains of Saccharomyces cereoisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cereoisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cereu isiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cereu isiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. |
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Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistanceFermentationStressesYeastsMicrosatelliteContaminantsStrains of Saccharomyces cereoisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cereoisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cereu isiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cereu isiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Carlos, Ctr Ciencias Agr, Dept Tecnol Agroind & Socioecon Rural, Lab Microbiol Agr & Mol, Araras, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Parasitol, Botucatu, SP, BrazilGenotyping Lab Biotecnol Ltd, Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias Botucatu, Dept Parasitol, Botucatu, SP, BrazilFAPESP: 2009/14617-4Soc Brasileira MicrobiologiaUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Genotyping Lab Biotecnol LtdReis, Vanda RenataBurlamaqui Faraco Antonangelo, Ana Teresa [UNESP]Guarnieri Bassi, Ana PaulaColombi, DeboraCeccato-Antonini, Sandra Regina2018-11-26T17:31:26Z2018-11-26T17:31:26Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article268-274application/pdfhttp://dx.doi.org/10.1016/j.bjm.2016.09.017Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 48, n. 2, p. 268-274, 2017.1517-8382http://hdl.handle.net/11449/16279910.1016/j.bjm.2016.09.017S1517-83822017000200268WOS:000401456800017S1517-83822017000200268.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiology0,630info:eu-repo/semantics/openAccess2024-01-05T06:23:45Zoai:repositorio.unesp.br:11449/162799Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:10:30.675171Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance |
title |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance |
spellingShingle |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance Reis, Vanda Renata Fermentation Stresses Yeasts Microsatellite Contaminants |
title_short |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance |
title_full |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance |
title_fullStr |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance |
title_full_unstemmed |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance |
title_sort |
Bioethanol strains of Saccharomyces cereuisiae characterised by microsatellite and stress resistance |
author |
Reis, Vanda Renata |
author_facet |
Reis, Vanda Renata Burlamaqui Faraco Antonangelo, Ana Teresa [UNESP] Guarnieri Bassi, Ana Paula Colombi, Debora Ceccato-Antonini, Sandra Regina |
author_role |
author |
author2 |
Burlamaqui Faraco Antonangelo, Ana Teresa [UNESP] Guarnieri Bassi, Ana Paula Colombi, Debora Ceccato-Antonini, Sandra Regina |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) Genotyping Lab Biotecnol Ltd |
dc.contributor.author.fl_str_mv |
Reis, Vanda Renata Burlamaqui Faraco Antonangelo, Ana Teresa [UNESP] Guarnieri Bassi, Ana Paula Colombi, Debora Ceccato-Antonini, Sandra Regina |
dc.subject.por.fl_str_mv |
Fermentation Stresses Yeasts Microsatellite Contaminants |
topic |
Fermentation Stresses Yeasts Microsatellite Contaminants |
description |
Strains of Saccharomyces cereoisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cereoisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cereu isiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The results obtained from the phenotypic tests by Principal-Component Analysis revealed that unlike the smooth colonies, the rough colonies of S. cereu isiae exhibit an enhanced resistance to stressful conditions resulting from the presence of excessive glucose and ethanol and high sedimentation rate. The microsatellite analysis was not successful to distinguish between the colony phenotypes as phenotypic assays. The relevant industrial strain PE-2 was observed in close genetic proximity to rough-colony although it does not display this colony morphology. A unique genetic pattern specific to a particular phenotype remains elusive. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-11-26T17:31:26Z 2018-11-26T17:31:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjm.2016.09.017 Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 48, n. 2, p. 268-274, 2017. 1517-8382 http://hdl.handle.net/11449/162799 10.1016/j.bjm.2016.09.017 S1517-83822017000200268 WOS:000401456800017 S1517-83822017000200268.pdf |
url |
http://dx.doi.org/10.1016/j.bjm.2016.09.017 http://hdl.handle.net/11449/162799 |
identifier_str_mv |
Brazilian Journal Of Microbiology. Sao Paulo: Soc Brasileira Microbiologia, v. 48, n. 2, p. 268-274, 2017. 1517-8382 10.1016/j.bjm.2016.09.017 S1517-83822017000200268 WOS:000401456800017 S1517-83822017000200268.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Microbiology 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
268-274 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
publisher.none.fl_str_mv |
Soc Brasileira Microbiologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129400234835968 |