Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production

Detalhes bibliográficos
Autor(a) principal: Saldanha,Roze L.
Data de Publicação: 2007
Outros Autores: Garcia,José E., Dekker,Robert F. H., Vilas-Boas,Laurival A., Barbosa,Aneli M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200013
Resumo: Nine isolates of Botryosphaeria spp. were evaluated for their growth and the production of cell wall-lytic enzymes (laccase, pectinase and beta-1,3-glucanase) when grown on basal medium in the absence and presence of the laccase inducer, veratryl alcohol (VA). The genetic relationship among the nine isolates collected from different host plants was determined by RAPD analyses. ITS sequence analysis showed eight closely related isolates classified as Botryosphaeria rhodina, and one isolate classified as Botryosphaeria ribis. RAPD analysis resolved the isolates into three main clusters based upon levels of laccase and beta-1,3-glucanase activity. There appears to be no correlation between pectinase production and genetic diversity among the nine isolates. However, the strain characterized as B. ribis, positioned out of the main cluster, was found to be the highest producer of pectinases in the presence of VA.
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spelling Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme productionBotryosphaeria isolatesITS and RAPDLaccasePectinasebeta-1,3-GlucanaseNine isolates of Botryosphaeria spp. were evaluated for their growth and the production of cell wall-lytic enzymes (laccase, pectinase and beta-1,3-glucanase) when grown on basal medium in the absence and presence of the laccase inducer, veratryl alcohol (VA). The genetic relationship among the nine isolates collected from different host plants was determined by RAPD analyses. ITS sequence analysis showed eight closely related isolates classified as Botryosphaeria rhodina, and one isolate classified as Botryosphaeria ribis. RAPD analysis resolved the isolates into three main clusters based upon levels of laccase and beta-1,3-glucanase activity. There appears to be no correlation between pectinase production and genetic diversity among the nine isolates. However, the strain characterized as B. ribis, positioned out of the main cluster, was found to be the highest producer of pectinases in the presence of VA.Sociedade Brasileira de Microbiologia2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200013Brazilian Journal of Microbiology v.38 n.2 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000200013info:eu-repo/semantics/openAccessSaldanha,Roze L.Garcia,José E.Dekker,Robert F. H.Vilas-Boas,Laurival A.Barbosa,Aneli M.eng2007-06-29T00:00:00Zoai:scielo:S1517-83822007000200013Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-06-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
title Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
spellingShingle Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
Saldanha,Roze L.
Botryosphaeria isolates
ITS and RAPD
Laccase
Pectinase
beta-1,3-Glucanase
title_short Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
title_full Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
title_fullStr Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
title_full_unstemmed Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
title_sort Genetic diversity among Botryosphaeria isolates and their correlation with cell wall-lytic enzyme production
author Saldanha,Roze L.
author_facet Saldanha,Roze L.
Garcia,José E.
Dekker,Robert F. H.
Vilas-Boas,Laurival A.
Barbosa,Aneli M.
author_role author
author2 Garcia,José E.
Dekker,Robert F. H.
Vilas-Boas,Laurival A.
Barbosa,Aneli M.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Saldanha,Roze L.
Garcia,José E.
Dekker,Robert F. H.
Vilas-Boas,Laurival A.
Barbosa,Aneli M.
dc.subject.por.fl_str_mv Botryosphaeria isolates
ITS and RAPD
Laccase
Pectinase
beta-1,3-Glucanase
topic Botryosphaeria isolates
ITS and RAPD
Laccase
Pectinase
beta-1,3-Glucanase
description Nine isolates of Botryosphaeria spp. were evaluated for their growth and the production of cell wall-lytic enzymes (laccase, pectinase and beta-1,3-glucanase) when grown on basal medium in the absence and presence of the laccase inducer, veratryl alcohol (VA). The genetic relationship among the nine isolates collected from different host plants was determined by RAPD analyses. ITS sequence analysis showed eight closely related isolates classified as Botryosphaeria rhodina, and one isolate classified as Botryosphaeria ribis. RAPD analysis resolved the isolates into three main clusters based upon levels of laccase and beta-1,3-glucanase activity. There appears to be no correlation between pectinase production and genetic diversity among the nine isolates. However, the strain characterized as B. ribis, positioned out of the main cluster, was found to be the highest producer of pectinases in the presence of VA.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000200013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.2 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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