Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100046 |
Resumo: | Phytate is the primary storage form of phosphate in plants. Monogastric animals like poultry, pigs and fishes have very low or no phytase activities in their digestive tracts therefore, are incapable to efficiently utilize phytate phosphorus from the feed. Phytase from microbial sources are supplemented to feedstuff of these to increase the uptake of phytate phosphorus. In the present work efforts were made to isolate and characterize proficient phytase producing fungi from soil. Phytase producing fungi were isolated using phytate specific medium. Fungal isolates were selected according to their higher phytase activities. These isolates were further characterized and identified by morphological and microscopic analysis and confirmed by amplification of 18S rRNA gene, using specific primers. This gene was subsequently sequenced and phylogenetic affiliations were assigned. Fungal isolates were identified as various species of Aspergillus. Phytases from these fungi could be utilized as a feed additive in poultry and swine industries. |
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Brazilian Journal of Microbiology |
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Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysisphytasephytatefungi18S rRNA geneAspergillusPhytate is the primary storage form of phosphate in plants. Monogastric animals like poultry, pigs and fishes have very low or no phytase activities in their digestive tracts therefore, are incapable to efficiently utilize phytate phosphorus from the feed. Phytase from microbial sources are supplemented to feedstuff of these to increase the uptake of phytate phosphorus. In the present work efforts were made to isolate and characterize proficient phytase producing fungi from soil. Phytase producing fungi were isolated using phytate specific medium. Fungal isolates were selected according to their higher phytase activities. These isolates were further characterized and identified by morphological and microscopic analysis and confirmed by amplification of 18S rRNA gene, using specific primers. This gene was subsequently sequenced and phylogenetic affiliations were assigned. Fungal isolates were identified as various species of Aspergillus. Phytases from these fungi could be utilized as a feed additive in poultry and swine industries.Sociedade Brasileira de Microbiologia2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100046Brazilian Journal of Microbiology v.44 n.1 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013005000021info:eu-repo/semantics/openAccessGontia-Mishra,ItiDeshmukh,DhanshreeTripathi,NirajBardiya-Bhurat,KhushbooTantwai,KeertiTiwari,Sharadeng2013-06-13T00:00:00Zoai:scielo:S1517-83822013000100046Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2013-06-13T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis |
title |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis |
spellingShingle |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis Gontia-Mishra,Iti phytase phytate fungi 18S rRNA gene Aspergillus |
title_short |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis |
title_full |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis |
title_fullStr |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis |
title_full_unstemmed |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis |
title_sort |
Isolation, morphological and molecular characterization of phytate-hydrolysing fungi by 18S rDNA sequence analysis |
author |
Gontia-Mishra,Iti |
author_facet |
Gontia-Mishra,Iti Deshmukh,Dhanshree Tripathi,Niraj Bardiya-Bhurat,Khushboo Tantwai,Keerti Tiwari,Sharad |
author_role |
author |
author2 |
Deshmukh,Dhanshree Tripathi,Niraj Bardiya-Bhurat,Khushboo Tantwai,Keerti Tiwari,Sharad |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gontia-Mishra,Iti Deshmukh,Dhanshree Tripathi,Niraj Bardiya-Bhurat,Khushboo Tantwai,Keerti Tiwari,Sharad |
dc.subject.por.fl_str_mv |
phytase phytate fungi 18S rRNA gene Aspergillus |
topic |
phytase phytate fungi 18S rRNA gene Aspergillus |
description |
Phytate is the primary storage form of phosphate in plants. Monogastric animals like poultry, pigs and fishes have very low or no phytase activities in their digestive tracts therefore, are incapable to efficiently utilize phytate phosphorus from the feed. Phytase from microbial sources are supplemented to feedstuff of these to increase the uptake of phytate phosphorus. In the present work efforts were made to isolate and characterize proficient phytase producing fungi from soil. Phytase producing fungi were isolated using phytate specific medium. Fungal isolates were selected according to their higher phytase activities. These isolates were further characterized and identified by morphological and microscopic analysis and confirmed by amplification of 18S rRNA gene, using specific primers. This gene was subsequently sequenced and phylogenetic affiliations were assigned. Fungal isolates were identified as various species of Aspergillus. Phytases from these fungi could be utilized as a feed additive in poultry and swine industries. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100046 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100046 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822013005000021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.44 n.1 2013 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122206003920896 |