In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil

Detalhes bibliográficos
Autor(a) principal: Aguiar,Daniel M.
Data de Publicação: 2008
Outros Autores: Hagiwara,Mitika K., Labruna,Marcelo B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014
Resumo: An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.
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spelling In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, BrazilEhrlichia canisDogMolecular CharacterizationEhrlichiosisAn Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300014info:eu-repo/semantics/openAccessAguiar,Daniel M.Hagiwara,Mitika K.Labruna,Marcelo B.eng2008-10-02T00:00:00Zoai:scielo:S1517-83822008000300014Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
title In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
spellingShingle In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
Aguiar,Daniel M.
Ehrlichia canis
Dog
Molecular Characterization
Ehrlichiosis
title_short In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
title_full In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
title_fullStr In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
title_full_unstemmed In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
title_sort In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
author Aguiar,Daniel M.
author_facet Aguiar,Daniel M.
Hagiwara,Mitika K.
Labruna,Marcelo B.
author_role author
author2 Hagiwara,Mitika K.
Labruna,Marcelo B.
author2_role author
author
dc.contributor.author.fl_str_mv Aguiar,Daniel M.
Hagiwara,Mitika K.
Labruna,Marcelo B.
dc.subject.por.fl_str_mv Ehrlichia canis
Dog
Molecular Characterization
Ehrlichiosis
topic Ehrlichia canis
Dog
Molecular Characterization
Ehrlichiosis
description An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.
publishDate 2008
dc.date.none.fl_str_mv 2008-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822008000300014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.39 n.3 2008
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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