In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014 |
Resumo: | An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil. |
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Brazilian Journal of Microbiology |
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In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, BrazilEhrlichia canisDogMolecular CharacterizationEhrlichiosisAn Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300014info:eu-repo/semantics/openAccessAguiar,Daniel M.Hagiwara,Mitika K.Labruna,Marcelo B.eng2008-10-02T00:00:00Zoai:scielo:S1517-83822008000300014Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil |
title |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil |
spellingShingle |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil Aguiar,Daniel M. Ehrlichia canis Dog Molecular Characterization Ehrlichiosis |
title_short |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil |
title_full |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil |
title_fullStr |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil |
title_full_unstemmed |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil |
title_sort |
In vitro isolation and molecular characterization of an Ehrlichia canis strain from São Paulo, Brazil |
author |
Aguiar,Daniel M. |
author_facet |
Aguiar,Daniel M. Hagiwara,Mitika K. Labruna,Marcelo B. |
author_role |
author |
author2 |
Hagiwara,Mitika K. Labruna,Marcelo B. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Aguiar,Daniel M. Hagiwara,Mitika K. Labruna,Marcelo B. |
dc.subject.por.fl_str_mv |
Ehrlichia canis Dog Molecular Characterization Ehrlichiosis |
topic |
Ehrlichia canis Dog Molecular Characterization Ehrlichiosis |
description |
An Ehrlichia canis isolate was obtained from an naturally infected dog exhibiting clinical signs of ehrlichiosis in São Paulo Municipality, state of São Paulo, Brazil. The isolate was characterized by PCR and DNA sequencing of portions of the ehrlichial genes dsb, 16SrRNA, and p28. Partial dsb and 16S rRNA sequences were identical to three and five other E. canis strains, respectively, from different countries and continents (including North America, Africa, Asia and Europe). Conversely, the p28 partial sequence for this E. canis (São Paulo) differed by 1, 2, and 2 nucleotides from the corresponding sequences of the E. canis strains Jake (from USA), Oklahoma (USA), and VHE (Venezuela), respectively. The results in this study indicate that E. canis is the only recognized Ehrlichia species infecting dogs in Brazil. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000300014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.3 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201820102656 |