Aseptic meningitis by echovirus 30 in São Paulo state, Brazil

Detalhes bibliográficos
Autor(a) principal: Machado,Bráulio Caetano
Data de Publicação: 2007
Outros Autores: Ferreira,Raquel de Souza, Carmona,Rita de Cássia Compagnoli, Timenetsky,Maria do Carmo Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100020
Resumo: The objective of this study was to identify and characterize the immunological and molecular aspects of Human Enterovirus (HEV) associated with aseptic meningitis cases in São Paulo State, Brazil. Clinical samples (cerebrospinal fluid CSF, stool and sera) were analyzed from 21 viral meningitis suspected cases collected in the period from 1998 to 1999. The isolated virus were identified as belonging to Enterovirus genus and specifically to echovirus serotype 30 (EV-30), using immunological techniques as Indirect Immunofluorescense Assay (IFA) and Neutralization (Nt) and by molecular technique of Reverse Transcription Polymerase Chain Reaction (RT-PCR) directed to the 5' NTR and VP1 regions of viral genome. The results obtained contributed with to the surveillance system of São Paulo State and they are important due to a shortage of epidemiological data about the circulation of EV-30 associated to aseptic meningitis in this region of the world.
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spelling Aseptic meningitis by echovirus 30 in São Paulo state, BrazilEnterovirusviral meningitismolecular and immunological diagnosisRT-PCR for Enterovirusindirect imunnofluorescense assayThe objective of this study was to identify and characterize the immunological and molecular aspects of Human Enterovirus (HEV) associated with aseptic meningitis cases in São Paulo State, Brazil. Clinical samples (cerebrospinal fluid CSF, stool and sera) were analyzed from 21 viral meningitis suspected cases collected in the period from 1998 to 1999. The isolated virus were identified as belonging to Enterovirus genus and specifically to echovirus serotype 30 (EV-30), using immunological techniques as Indirect Immunofluorescense Assay (IFA) and Neutralization (Nt) and by molecular technique of Reverse Transcription Polymerase Chain Reaction (RT-PCR) directed to the 5' NTR and VP1 regions of viral genome. The results obtained contributed with to the surveillance system of São Paulo State and they are important due to a shortage of epidemiological data about the circulation of EV-30 associated to aseptic meningitis in this region of the world.Sociedade Brasileira de Microbiologia2007-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100020Brazilian Journal of Microbiology v.38 n.1 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000100020info:eu-repo/semantics/openAccessMachado,Bráulio CaetanoFerreira,Raquel de SouzaCarmona,Rita de Cássia CompagnoliTimenetsky,Maria do Carmo Sampaio Tavareseng2007-04-24T00:00:00Zoai:scielo:S1517-83822007000100020Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-04-24T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
title Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
spellingShingle Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
Machado,Bráulio Caetano
Enterovirus
viral meningitis
molecular and immunological diagnosis
RT-PCR for Enterovirus
indirect imunnofluorescense assay
title_short Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
title_full Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
title_fullStr Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
title_full_unstemmed Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
title_sort Aseptic meningitis by echovirus 30 in São Paulo state, Brazil
author Machado,Bráulio Caetano
author_facet Machado,Bráulio Caetano
Ferreira,Raquel de Souza
Carmona,Rita de Cássia Compagnoli
Timenetsky,Maria do Carmo Sampaio Tavares
author_role author
author2 Ferreira,Raquel de Souza
Carmona,Rita de Cássia Compagnoli
Timenetsky,Maria do Carmo Sampaio Tavares
author2_role author
author
author
dc.contributor.author.fl_str_mv Machado,Bráulio Caetano
Ferreira,Raquel de Souza
Carmona,Rita de Cássia Compagnoli
Timenetsky,Maria do Carmo Sampaio Tavares
dc.subject.por.fl_str_mv Enterovirus
viral meningitis
molecular and immunological diagnosis
RT-PCR for Enterovirus
indirect imunnofluorescense assay
topic Enterovirus
viral meningitis
molecular and immunological diagnosis
RT-PCR for Enterovirus
indirect imunnofluorescense assay
description The objective of this study was to identify and characterize the immunological and molecular aspects of Human Enterovirus (HEV) associated with aseptic meningitis cases in São Paulo State, Brazil. Clinical samples (cerebrospinal fluid CSF, stool and sera) were analyzed from 21 viral meningitis suspected cases collected in the period from 1998 to 1999. The isolated virus were identified as belonging to Enterovirus genus and specifically to echovirus serotype 30 (EV-30), using immunological techniques as Indirect Immunofluorescense Assay (IFA) and Neutralization (Nt) and by molecular technique of Reverse Transcription Polymerase Chain Reaction (RT-PCR) directed to the 5' NTR and VP1 regions of viral genome. The results obtained contributed with to the surveillance system of São Paulo State and they are important due to a shortage of epidemiological data about the circulation of EV-30 associated to aseptic meningitis in this region of the world.
publishDate 2007
dc.date.none.fl_str_mv 2007-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000100020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.1 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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