Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages

Detalhes bibliográficos
Autor(a) principal: Sawitzki,Maristela Cortez
Data de Publicação: 2007
Outros Autores: Fiorentini,Ângela Maria, Brod,Fábio Cristiano Angonesi, Tagliari,Caroline, Bertol,Teresinha Marisa, Arisi,Ana Carolina Maisonnave, Sant'Anna,Ernani Sebastião
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300031
Resumo: The purpose of the present work was to characterize promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented artisanal sausage manufactured in the northwestern region of Rio Grande do Sul state, Brazil. From 127 isolates of homofermentative, Gram-positive and catalase-negative lactic acid bacteria, ten isolates were randomly selected and the phenotypic characterization and species-specific PCR were performed. Genomic DNA from each isolated strain and from the reference strains L. plantarum ATCC 8014 and L. pentosus ATCC 8041 were amplified using two pairs of L. plantarum species-specific primers (16/Lpl and LbP11/LbP12). The results of the phenotypic characterization and species-specific PCR indicated that five out of ten isolates were Lactobacillus plantarum.
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spelling Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausagesfermented sausageL. plantarumphenotypic characterizationPCRThe purpose of the present work was to characterize promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented artisanal sausage manufactured in the northwestern region of Rio Grande do Sul state, Brazil. From 127 isolates of homofermentative, Gram-positive and catalase-negative lactic acid bacteria, ten isolates were randomly selected and the phenotypic characterization and species-specific PCR were performed. Genomic DNA from each isolated strain and from the reference strains L. plantarum ATCC 8014 and L. pentosus ATCC 8041 were amplified using two pairs of L. plantarum species-specific primers (16/Lpl and LbP11/LbP12). The results of the phenotypic characterization and species-specific PCR indicated that five out of ten isolates were Lactobacillus plantarum.Sociedade Brasileira de Microbiologia2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300031Brazilian Journal of Microbiology v.38 n.3 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000300031info:eu-repo/semantics/openAccessSawitzki,Maristela CortezFiorentini,Ângela MariaBrod,Fábio Cristiano AngonesiTagliari,CarolineBertol,Teresinha MarisaArisi,Ana Carolina MaisonnaveSant'Anna,Ernani Sebastiãoeng2007-10-17T00:00:00Zoai:scielo:S1517-83822007000300031Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-10-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
title Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
spellingShingle Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
Sawitzki,Maristela Cortez
fermented sausage
L. plantarum
phenotypic characterization
PCR
title_short Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
title_full Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
title_fullStr Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
title_full_unstemmed Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
title_sort Phenotypic characterization and species-specific PCR of promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented sausages
author Sawitzki,Maristela Cortez
author_facet Sawitzki,Maristela Cortez
Fiorentini,Ângela Maria
Brod,Fábio Cristiano Angonesi
Tagliari,Caroline
Bertol,Teresinha Marisa
Arisi,Ana Carolina Maisonnave
Sant'Anna,Ernani Sebastião
author_role author
author2 Fiorentini,Ângela Maria
Brod,Fábio Cristiano Angonesi
Tagliari,Caroline
Bertol,Teresinha Marisa
Arisi,Ana Carolina Maisonnave
Sant'Anna,Ernani Sebastião
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sawitzki,Maristela Cortez
Fiorentini,Ângela Maria
Brod,Fábio Cristiano Angonesi
Tagliari,Caroline
Bertol,Teresinha Marisa
Arisi,Ana Carolina Maisonnave
Sant'Anna,Ernani Sebastião
dc.subject.por.fl_str_mv fermented sausage
L. plantarum
phenotypic characterization
PCR
topic fermented sausage
L. plantarum
phenotypic characterization
PCR
description The purpose of the present work was to characterize promising starter culture strains of Lactobacillus plantarum isolated from naturally fermented artisanal sausage manufactured in the northwestern region of Rio Grande do Sul state, Brazil. From 127 isolates of homofermentative, Gram-positive and catalase-negative lactic acid bacteria, ten isolates were randomly selected and the phenotypic characterization and species-specific PCR were performed. Genomic DNA from each isolated strain and from the reference strains L. plantarum ATCC 8014 and L. pentosus ATCC 8041 were amplified using two pairs of L. plantarum species-specific primers (16/Lpl and LbP11/LbP12). The results of the phenotypic characterization and species-specific PCR indicated that five out of ten isolates were Lactobacillus plantarum.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300031
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000300031
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.3 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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