Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis

Detalhes bibliográficos
Autor(a) principal: Gibotti,Adriana
Data de Publicação: 2004
Outros Autores: Tanaka,Tânia L., Oliveira,Valéria R., Taddei,Carla R., Martinez,Marina B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012
Resumo: In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.
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spelling Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysisipaenteroinvasive Escherichia colipolymorphismPCR-RFLPIn this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.Sociedade Brasileira de Microbiologia2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012Brazilian Journal of Microbiology v.35 n.1-2 2004reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822004000100012info:eu-repo/semantics/openAccessGibotti,AdrianaTanaka,Tânia L.Oliveira,Valéria R.Taddei,Carla R.Martinez,Marina B.eng2004-11-16T00:00:00Zoai:scielo:S1517-83822004000100012Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
spellingShingle Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
Gibotti,Adriana
ipa
enteroinvasive Escherichia coli
polymorphism
PCR-RFLP
title_short Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_full Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_fullStr Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_full_unstemmed Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
title_sort Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis
author Gibotti,Adriana
author_facet Gibotti,Adriana
Tanaka,Tânia L.
Oliveira,Valéria R.
Taddei,Carla R.
Martinez,Marina B.
author_role author
author2 Tanaka,Tânia L.
Oliveira,Valéria R.
Taddei,Carla R.
Martinez,Marina B.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Gibotti,Adriana
Tanaka,Tânia L.
Oliveira,Valéria R.
Taddei,Carla R.
Martinez,Marina B.
dc.subject.por.fl_str_mv ipa
enteroinvasive Escherichia coli
polymorphism
PCR-RFLP
topic ipa
enteroinvasive Escherichia coli
polymorphism
PCR-RFLP
description In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822004000100012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.35 n.1-2 2004
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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