Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems

• Autor(a) principal: Yamaguti,M.
• Data de Publicação: 2008
• Outros Autores: Muller,E.E., Piffer,A.I., Kich,J.D., Klein,C.S., Kuchiishi,S.S.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300011
• topic: Swine Enzootic Pneumonia; Mycoplasma hyopneumoniae; diagnosis; Nested- PCR

Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport media, and DNA extraction methods, using eight pigs. Based on the optimization results, a second experiment was conducted for testing validity using 40 animals. In conclusion, the obtained results of the N-PCR optimization and validation allow us to recommend this test as a routine monitoring diagnostic method for Mycoplasma hyopneumoniae infection in swine herds.