Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse

Detalhes bibliográficos
Autor(a) principal: Rezende,Maria Inês
Data de Publicação: 2002
Outros Autores: Barbosa,Aneli de Melo, Vasconcelos,Ana Flora Dalberto, Endo,Asae Sakurada
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014
Resumo: Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and sugarcane bagasse concentration, on xylanase production were also investigated. The growth profile of Trichoderma harzianum was followed over 20 days on 14% (w/v) bagasse, and highest xylanase activity (288U/ml) appeared on the seventh day. The enzymatic extract after precipitation with ammonium sulphate was submitted to electrophoresis on polyacrylamide gels and showed 4 protein-staining bands, one of which exhibited xylanase activity.
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spelling Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasseTrichoderma harzianumxylanasesolid state fermentationsugarcane bagasseSugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and sugarcane bagasse concentration, on xylanase production were also investigated. The growth profile of Trichoderma harzianum was followed over 20 days on 14% (w/v) bagasse, and highest xylanase activity (288U/ml) appeared on the seventh day. The enzymatic extract after precipitation with ammonium sulphate was submitted to electrophoresis on polyacrylamide gels and showed 4 protein-staining bands, one of which exhibited xylanase activity.Sociedade Brasileira de Microbiologia2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014Brazilian Journal of Microbiology v.33 n.1 2002reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822002000100014info:eu-repo/semantics/openAccessRezende,Maria InêsBarbosa,Aneli de MeloVasconcelos,Ana Flora DalbertoEndo,Asae Sakuradaeng2002-09-16T00:00:00Zoai:scielo:S1517-83822002000100014Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2002-09-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
title Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
spellingShingle Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
Rezende,Maria Inês
Trichoderma harzianum
xylanase
solid state fermentation
sugarcane bagasse
title_short Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
title_full Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
title_fullStr Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
title_full_unstemmed Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
title_sort Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse
author Rezende,Maria Inês
author_facet Rezende,Maria Inês
Barbosa,Aneli de Melo
Vasconcelos,Ana Flora Dalberto
Endo,Asae Sakurada
author_role author
author2 Barbosa,Aneli de Melo
Vasconcelos,Ana Flora Dalberto
Endo,Asae Sakurada
author2_role author
author
author
dc.contributor.author.fl_str_mv Rezende,Maria Inês
Barbosa,Aneli de Melo
Vasconcelos,Ana Flora Dalberto
Endo,Asae Sakurada
dc.subject.por.fl_str_mv Trichoderma harzianum
xylanase
solid state fermentation
sugarcane bagasse
topic Trichoderma harzianum
xylanase
solid state fermentation
sugarcane bagasse
description Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and sugarcane bagasse concentration, on xylanase production were also investigated. The growth profile of Trichoderma harzianum was followed over 20 days on 14% (w/v) bagasse, and highest xylanase activity (288U/ml) appeared on the seventh day. The enzymatic extract after precipitation with ammonium sulphate was submitted to electrophoresis on polyacrylamide gels and showed 4 protein-staining bands, one of which exhibited xylanase activity.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822002000100014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.33 n.1 2002
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
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reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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