Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.indcrop.2018.05.022 http://hdl.handle.net/11449/176359 |
Resumo: | Endophytic fungi are widely studied as producers of secondary metabolites of biotechnological interest. In recent years, the interest in these fungi as new sources of enzymes, especially hydrolytic, has increased. In the present study, 14 strains of endophytic fungi not yet explored as enzymes sources were randomly chosen and prospected for cellulases and xylanases production by solid-state fermentation. Initially, fungi were cultivated in a mixture (1:1 w/w) of sugarcane bagasse and wheat bran for 7 days, at 28 °C. In this initial screening, 4 fungi excelled in endoglucanase activity (U/g): Cladosporium cladosporioides PAJ 03 (88.51 ± 1.0), Phomopsis stipata SC 04 (83.44 ± 7.7), Trichoderma viridae PAJ 01 (64.56 ± 4.0) and Botryosphaeria sp. AM 01 (42.79 ± 1.6). On the other hand, the following 4 fungi stood out in relation to β-glucosidase activity (U/g): Saccharicola sp. EJC 04 (51.56 ± 2.7), Paecilomyces sp. SF 021 (33.19 ± 9.2), Ustilaginoidea sp. CV 04 (29.75 ± 0.8) and Ustilaginoidea sp. XYA 04 (21.72 ± 3.05). Among these fungi, P. stipata SC 04 and Botryosphaeria sp. AM 01 were the best producers of xylanase and β-xilosidase (694,33 and 4,87 U/g, respectively). These 8 fungi were then cultured in new mixtures (1:1 w/w) of lignocellulosic substrates. Botryosphaeria sp. AM01and Saccharicola sp. EJC04 stood out regarding endoglucanase and β-glucosidase activities (184.74 ± 6.0 and 92.28 ± 9.57 U/g, respectively) when cultivated on cotton seed meal and wheat bran and were selected to continue the study. The influence of time cultivation, inoculum amount and substrate initial moisture content was evaluated and the best condition for cellulases production was 192 h, six mycelial plugs and 65%, respectively, for both fungi. Cellulases and xylanases produced under these conditions were characterized and optimum pH and temperature values were between 4.5–6 and 60–75 °C, respectively. The enzymes were stable over a wide pH range and under 30–70 °C. β-glucosidase from both isolates retained about 75–80% of their activity in the presence of glucose at 6 mM. The presence of ethanol stimulated β-glucosidase activity from Botryosphaeria sp. AM01 (about 60% higher in the presence of ethanol at 15%). On the other hand, the activity of β-glucosidase produced by Saccharicola sp. EJC 04 was reduced at ethanol concentrations above 15%. A blend of the enzymatic extracts was used to saccharify pretreated sugarcane bagasse and a face-centered central composite design was used to find the best conditions. Under the predicted optimum condition (50 °C, 5% of sugarcane bagasse, 150 U g−1 of endoglucanase and 20 h), glucose and xylose concentrations obtained were 3.56 and 1.66 mg mL−1, respectively. These results show that the 14 endophytic fungi studied have potential to be explored as producers of plant material degrading enzymes. Botryosphaeria sp. AM01 and Saccharicola sp. EJC 04 are promising in relation to the production of cellulases and xylanases and their enzymatic extracts have potential for application in pre-treated sugarcane bagasse saccharification processes. |
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Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasseCellulasesEndophytic fungiSolid state fermentationSugarcane bagasse saccharificationXylanasesEndophytic fungi are widely studied as producers of secondary metabolites of biotechnological interest. In recent years, the interest in these fungi as new sources of enzymes, especially hydrolytic, has increased. In the present study, 14 strains of endophytic fungi not yet explored as enzymes sources were randomly chosen and prospected for cellulases and xylanases production by solid-state fermentation. Initially, fungi were cultivated in a mixture (1:1 w/w) of sugarcane bagasse and wheat bran for 7 days, at 28 °C. In this initial screening, 4 fungi excelled in endoglucanase activity (U/g): Cladosporium cladosporioides PAJ 03 (88.51 ± 1.0), Phomopsis stipata SC 04 (83.44 ± 7.7), Trichoderma viridae PAJ 01 (64.56 ± 4.0) and Botryosphaeria sp. AM 01 (42.79 ± 1.6). On the other hand, the following 4 fungi stood out in relation to β-glucosidase activity (U/g): Saccharicola sp. EJC 04 (51.56 ± 2.7), Paecilomyces sp. SF 021 (33.19 ± 9.2), Ustilaginoidea sp. CV 04 (29.75 ± 0.8) and Ustilaginoidea sp. XYA 04 (21.72 ± 3.05). Among these fungi, P. stipata SC 04 and Botryosphaeria sp. AM 01 were the best producers of xylanase and β-xilosidase (694,33 and 4,87 U/g, respectively). These 8 fungi were then cultured in new mixtures (1:1 w/w) of lignocellulosic substrates. Botryosphaeria sp. AM01and Saccharicola sp. EJC04 stood out regarding endoglucanase and β-glucosidase activities (184.74 ± 6.0 and 92.28 ± 9.57 U/g, respectively) when cultivated on cotton seed meal and wheat bran and were selected to continue the study. The influence of time cultivation, inoculum amount and substrate initial moisture content was evaluated and the best condition for cellulases production was 192 h, six mycelial plugs and 65%, respectively, for both fungi. Cellulases and xylanases produced under these conditions were characterized and optimum pH and temperature values were between 4.5–6 and 60–75 °C, respectively. The enzymes were stable over a wide pH range and under 30–70 °C. β-glucosidase from both isolates retained about 75–80% of their activity in the presence of glucose at 6 mM. The presence of ethanol stimulated β-glucosidase activity from Botryosphaeria sp. AM01 (about 60% higher in the presence of ethanol at 15%). On the other hand, the activity of β-glucosidase produced by Saccharicola sp. EJC 04 was reduced at ethanol concentrations above 15%. A blend of the enzymatic extracts was used to saccharify pretreated sugarcane bagasse and a face-centered central composite design was used to find the best conditions. Under the predicted optimum condition (50 °C, 5% of sugarcane bagasse, 150 U g−1 of endoglucanase and 20 h), glucose and xylose concentrations obtained were 3.56 and 1.66 mg mL−1, respectively. These results show that the 14 endophytic fungi studied have potential to be explored as producers of plant material degrading enzymes. Botryosphaeria sp. AM01 and Saccharicola sp. EJC 04 are promising in relation to the production of cellulases and xylanases and their enzymatic extracts have potential for application in pre-treated sugarcane bagasse saccharification processes.Department of Biochemistry and Chemical Technology IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55Department of Biology IBILCE/UNESP–Univ Estadual Paulista Rua Cristóvão Colombo, 2265, São José do Rio PretoDepartment of Chemistry and Environmental Sciences IBILCE/UNESP – Univ Estadual Paulista, Rua Cristóvão Colombo, 2265, São José do Rio PretoDepartment of Organic Chemistry IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55Department of Biochemistry and Microbiology IB/UNESP–Univ Estadual Paulista, Avenida 24A, 1515Department of Biochemistry and Chemical Technology IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55Department of Biology IBILCE/UNESP–Univ Estadual Paulista Rua Cristóvão Colombo, 2265, São José do Rio PretoDepartment of Chemistry and Environmental Sciences IBILCE/UNESP – Univ Estadual Paulista, Rua Cristóvão Colombo, 2265, São José do Rio PretoDepartment of Organic Chemistry IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55Department of Biochemistry and Microbiology IB/UNESP–Univ Estadual Paulista, Avenida 24A, 1515Universidade Estadual Paulista (Unesp)Marques, Natália Paganini [UNESP]de Cassia Pereira, Josiani [UNESP]Gomes, Eleni [UNESP]da Silva, Roberto [UNESP]Araújo, Angela Regina [UNESP]Ferreira, Henrique [UNESP]Rodrigues, André [UNESP]Dussán, Kelly Johana [UNESP]Bocchini, Daniela Alonso [UNESP]2018-12-11T17:20:28Z2018-12-11T17:20:28Z2018-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article66-75application/pdfhttp://dx.doi.org/10.1016/j.indcrop.2018.05.022Industrial Crops and Products, v. 122, p. 66-75.0926-6690http://hdl.handle.net/11449/17635910.1016/j.indcrop.2018.05.0222-s2.0-850473863882-s2.0-85047386388.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIndustrial Crops and Products1,091info:eu-repo/semantics/openAccess2023-11-23T06:15:06Zoai:repositorio.unesp.br:11449/176359Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-23T06:15:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse |
title |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse |
spellingShingle |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse Marques, Natália Paganini [UNESP] Cellulases Endophytic fungi Solid state fermentation Sugarcane bagasse saccharification Xylanases |
title_short |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse |
title_full |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse |
title_fullStr |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse |
title_full_unstemmed |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse |
title_sort |
Cellulases and xylanases production by endophytic fungi by solid state fermentation using lignocellulosic substrates and enzymatic saccharification of pretreated sugarcane bagasse |
author |
Marques, Natália Paganini [UNESP] |
author_facet |
Marques, Natália Paganini [UNESP] de Cassia Pereira, Josiani [UNESP] Gomes, Eleni [UNESP] da Silva, Roberto [UNESP] Araújo, Angela Regina [UNESP] Ferreira, Henrique [UNESP] Rodrigues, André [UNESP] Dussán, Kelly Johana [UNESP] Bocchini, Daniela Alonso [UNESP] |
author_role |
author |
author2 |
de Cassia Pereira, Josiani [UNESP] Gomes, Eleni [UNESP] da Silva, Roberto [UNESP] Araújo, Angela Regina [UNESP] Ferreira, Henrique [UNESP] Rodrigues, André [UNESP] Dussán, Kelly Johana [UNESP] Bocchini, Daniela Alonso [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Marques, Natália Paganini [UNESP] de Cassia Pereira, Josiani [UNESP] Gomes, Eleni [UNESP] da Silva, Roberto [UNESP] Araújo, Angela Regina [UNESP] Ferreira, Henrique [UNESP] Rodrigues, André [UNESP] Dussán, Kelly Johana [UNESP] Bocchini, Daniela Alonso [UNESP] |
dc.subject.por.fl_str_mv |
Cellulases Endophytic fungi Solid state fermentation Sugarcane bagasse saccharification Xylanases |
topic |
Cellulases Endophytic fungi Solid state fermentation Sugarcane bagasse saccharification Xylanases |
description |
Endophytic fungi are widely studied as producers of secondary metabolites of biotechnological interest. In recent years, the interest in these fungi as new sources of enzymes, especially hydrolytic, has increased. In the present study, 14 strains of endophytic fungi not yet explored as enzymes sources were randomly chosen and prospected for cellulases and xylanases production by solid-state fermentation. Initially, fungi were cultivated in a mixture (1:1 w/w) of sugarcane bagasse and wheat bran for 7 days, at 28 °C. In this initial screening, 4 fungi excelled in endoglucanase activity (U/g): Cladosporium cladosporioides PAJ 03 (88.51 ± 1.0), Phomopsis stipata SC 04 (83.44 ± 7.7), Trichoderma viridae PAJ 01 (64.56 ± 4.0) and Botryosphaeria sp. AM 01 (42.79 ± 1.6). On the other hand, the following 4 fungi stood out in relation to β-glucosidase activity (U/g): Saccharicola sp. EJC 04 (51.56 ± 2.7), Paecilomyces sp. SF 021 (33.19 ± 9.2), Ustilaginoidea sp. CV 04 (29.75 ± 0.8) and Ustilaginoidea sp. XYA 04 (21.72 ± 3.05). Among these fungi, P. stipata SC 04 and Botryosphaeria sp. AM 01 were the best producers of xylanase and β-xilosidase (694,33 and 4,87 U/g, respectively). These 8 fungi were then cultured in new mixtures (1:1 w/w) of lignocellulosic substrates. Botryosphaeria sp. AM01and Saccharicola sp. EJC04 stood out regarding endoglucanase and β-glucosidase activities (184.74 ± 6.0 and 92.28 ± 9.57 U/g, respectively) when cultivated on cotton seed meal and wheat bran and were selected to continue the study. The influence of time cultivation, inoculum amount and substrate initial moisture content was evaluated and the best condition for cellulases production was 192 h, six mycelial plugs and 65%, respectively, for both fungi. Cellulases and xylanases produced under these conditions were characterized and optimum pH and temperature values were between 4.5–6 and 60–75 °C, respectively. The enzymes were stable over a wide pH range and under 30–70 °C. β-glucosidase from both isolates retained about 75–80% of their activity in the presence of glucose at 6 mM. The presence of ethanol stimulated β-glucosidase activity from Botryosphaeria sp. AM01 (about 60% higher in the presence of ethanol at 15%). On the other hand, the activity of β-glucosidase produced by Saccharicola sp. EJC 04 was reduced at ethanol concentrations above 15%. A blend of the enzymatic extracts was used to saccharify pretreated sugarcane bagasse and a face-centered central composite design was used to find the best conditions. Under the predicted optimum condition (50 °C, 5% of sugarcane bagasse, 150 U g−1 of endoglucanase and 20 h), glucose and xylose concentrations obtained were 3.56 and 1.66 mg mL−1, respectively. These results show that the 14 endophytic fungi studied have potential to be explored as producers of plant material degrading enzymes. Botryosphaeria sp. AM01 and Saccharicola sp. EJC 04 are promising in relation to the production of cellulases and xylanases and their enzymatic extracts have potential for application in pre-treated sugarcane bagasse saccharification processes. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:20:28Z 2018-12-11T17:20:28Z 2018-10-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.indcrop.2018.05.022 Industrial Crops and Products, v. 122, p. 66-75. 0926-6690 http://hdl.handle.net/11449/176359 10.1016/j.indcrop.2018.05.022 2-s2.0-85047386388 2-s2.0-85047386388.pdf |
url |
http://dx.doi.org/10.1016/j.indcrop.2018.05.022 http://hdl.handle.net/11449/176359 |
identifier_str_mv |
Industrial Crops and Products, v. 122, p. 66-75. 0926-6690 10.1016/j.indcrop.2018.05.022 2-s2.0-85047386388 2-s2.0-85047386388.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Industrial Crops and Products 1,091 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
66-75 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965043829243904 |