Leptospirosis diagnosis using Nested-PCR
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031 |
Resumo: | Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR method for diagnosis of leptospirosis. Primers were designed to amplify a 264 bp region within the LipL32 gene. The sensitivity and specificity of the assay was evaluated using seven saprophytic serovars and 35 pathogenic serovars. This technique showed to be very specific for pathogenic serovars, however it lacked sensitivity. In order to enhance the sensitivity, another primer pair was designed which amplify a 183 bp region within the 264 bp region in lipL32 gene, and used in a Nested-PCR assay. This approach was much more sensitive than traditional PCR. |
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Brazilian Journal of Microbiology |
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Leptospirosis diagnosis using Nested-PCRLeptospirosisPCRLeptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR method for diagnosis of leptospirosis. Primers were designed to amplify a 264 bp region within the LipL32 gene. The sensitivity and specificity of the assay was evaluated using seven saprophytic serovars and 35 pathogenic serovars. This technique showed to be very specific for pathogenic serovars, however it lacked sensitivity. In order to enhance the sensitivity, another primer pair was designed which amplify a 183 bp region within the 264 bp region in lipL32 gene, and used in a Nested-PCR assay. This approach was much more sensitive than traditional PCR.Sociedade Brasileira de Microbiologia2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031Brazilian Journal of Microbiology v.34 suppl.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000500031info:eu-repo/semantics/openAccessNassi,FernandaSeixas,Fabiana KömmlingJouglard,Sandra Denize DornelesSimionatto,SimoneSilva,Everton FagondeSeyffert,NúbiaBrod,Claudiomar SoaresDellagostin,Odir Antonioeng2004-11-29T00:00:00Zoai:scielo:S1517-83822003000500031Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Leptospirosis diagnosis using Nested-PCR |
title |
Leptospirosis diagnosis using Nested-PCR |
spellingShingle |
Leptospirosis diagnosis using Nested-PCR Nassi,Fernanda Leptospirosis PCR |
title_short |
Leptospirosis diagnosis using Nested-PCR |
title_full |
Leptospirosis diagnosis using Nested-PCR |
title_fullStr |
Leptospirosis diagnosis using Nested-PCR |
title_full_unstemmed |
Leptospirosis diagnosis using Nested-PCR |
title_sort |
Leptospirosis diagnosis using Nested-PCR |
author |
Nassi,Fernanda |
author_facet |
Nassi,Fernanda Seixas,Fabiana Kömmling Jouglard,Sandra Denize Dorneles Simionatto,Simone Silva,Everton Fagonde Seyffert,Núbia Brod,Claudiomar Soares Dellagostin,Odir Antonio |
author_role |
author |
author2 |
Seixas,Fabiana Kömmling Jouglard,Sandra Denize Dorneles Simionatto,Simone Silva,Everton Fagonde Seyffert,Núbia Brod,Claudiomar Soares Dellagostin,Odir Antonio |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nassi,Fernanda Seixas,Fabiana Kömmling Jouglard,Sandra Denize Dorneles Simionatto,Simone Silva,Everton Fagonde Seyffert,Núbia Brod,Claudiomar Soares Dellagostin,Odir Antonio |
dc.subject.por.fl_str_mv |
Leptospirosis PCR |
topic |
Leptospirosis PCR |
description |
Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods. The aim of this work was to evaluate and optimize a Nested-PCR method for diagnosis of leptospirosis. Primers were designed to amplify a 264 bp region within the LipL32 gene. The sensitivity and specificity of the assay was evaluated using seven saprophytic serovars and 35 pathogenic serovars. This technique showed to be very specific for pathogenic serovars, however it lacked sensitivity. In order to enhance the sensitivity, another primer pair was designed which amplify a 183 bp region within the 264 bp region in lipL32 gene, and used in a Nested-PCR assay. This approach was much more sensitive than traditional PCR. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500031 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822003000500031 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.34 suppl.1 2003 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199774330880 |