Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method

Detalhes bibliográficos
Autor(a) principal: Monte,Leonardo Garcia
Data de Publicação: 2012
Outros Autores: Jorge,Sérgio, Luiz,João Paulo Mesquita, Sinnott,Francine, Seixas,Fabiana Kömmling, Aleixo,José Antonio Guimarães, Samartino,Luis Ernesto, Conceição,Fabricio Rochedo, Hartleben,Cláudia Pinho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200023
Resumo: Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
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spelling Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR methodCanine leptospirosisIMS-PCRLipL32Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.Sociedade Brasileira de Microbiologia2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200023Brazilian Journal of Microbiology v.43 n.2 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000200023info:eu-repo/semantics/openAccessMonte,Leonardo GarciaJorge,SérgioLuiz,João Paulo MesquitaSinnott,FrancineSeixas,Fabiana KömmlingAleixo,José Antonio GuimarãesSamartino,Luis ErnestoConceição,Fabricio RochedoHartleben,Cláudia Pinhoeng2012-08-07T00:00:00Zoai:scielo:S1517-83822012000200023Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-08-07T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
spellingShingle Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
Monte,Leonardo Garcia
Canine leptospirosis
IMS-PCR
LipL32
title_short Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_full Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_fullStr Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_full_unstemmed Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_sort Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
author Monte,Leonardo Garcia
author_facet Monte,Leonardo Garcia
Jorge,Sérgio
Luiz,João Paulo Mesquita
Sinnott,Francine
Seixas,Fabiana Kömmling
Aleixo,José Antonio Guimarães
Samartino,Luis Ernesto
Conceição,Fabricio Rochedo
Hartleben,Cláudia Pinho
author_role author
author2 Jorge,Sérgio
Luiz,João Paulo Mesquita
Sinnott,Francine
Seixas,Fabiana Kömmling
Aleixo,José Antonio Guimarães
Samartino,Luis Ernesto
Conceição,Fabricio Rochedo
Hartleben,Cláudia Pinho
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Monte,Leonardo Garcia
Jorge,Sérgio
Luiz,João Paulo Mesquita
Sinnott,Francine
Seixas,Fabiana Kömmling
Aleixo,José Antonio Guimarães
Samartino,Luis Ernesto
Conceição,Fabricio Rochedo
Hartleben,Cláudia Pinho
dc.subject.por.fl_str_mv Canine leptospirosis
IMS-PCR
LipL32
topic Canine leptospirosis
IMS-PCR
LipL32
description Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822012000200023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.43 n.2 2012
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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