Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design

Detalhes bibliográficos
Autor(a) principal: Moghannem,Saad A.M.
Data de Publicação: 2018
Outros Autores: Farag,Mohamed M.S., Shehab,Amr M., Azab,Mohamed S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300452
Resumo: Abstract Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography-mass spectroscopy (GC-MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.
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spelling Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental designBacterial exopolysaccharidePlackett-BurmanBox-BehnkenDEAE-celluloseAbstract Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography-mass spectroscopy (GC-MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.Sociedade Brasileira de Microbiologia2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300452Brazilian Journal of Microbiology v.49 n.3 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2017.05.012info:eu-repo/semantics/openAccessMoghannem,Saad A.M.Farag,Mohamed M.S.Shehab,Amr M.Azab,Mohamed S.eng2018-08-03T00:00:00Zoai:scielo:S1517-83822018000300452Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-08-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
spellingShingle Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
Moghannem,Saad A.M.
Bacterial exopolysaccharide
Plackett-Burman
Box-Behnken
DEAE-cellulose
title_short Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_full Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_fullStr Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_full_unstemmed Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
title_sort Exopolysaccharide production from Bacillus velezensis KY471306 using statistical experimental design
author Moghannem,Saad A.M.
author_facet Moghannem,Saad A.M.
Farag,Mohamed M.S.
Shehab,Amr M.
Azab,Mohamed S.
author_role author
author2 Farag,Mohamed M.S.
Shehab,Amr M.
Azab,Mohamed S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Moghannem,Saad A.M.
Farag,Mohamed M.S.
Shehab,Amr M.
Azab,Mohamed S.
dc.subject.por.fl_str_mv Bacterial exopolysaccharide
Plackett-Burman
Box-Behnken
DEAE-cellulose
topic Bacterial exopolysaccharide
Plackett-Burman
Box-Behnken
DEAE-cellulose
description Abstract Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters. Box-Behnken RSM indicated that; the optimum concentration for each parameter was 12% (w/v) for molasses, 6 g/L yeast extract and 30 °C for incubation temperature. The most potent bacterial isolate was identified as Bacillus velezensis KY498625. After production, EPS was extracted, purified using DEAE-cellulose, identified using Fourier transform infrared (FTIR), gel permeation chromatography (GPC) and gas chromatography-mass spectroscopy (GC-MS). The result indicated that; it has molecular weight 1.14 × 105 D consisting of glucose, mannose and galactose.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300452
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300452
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2017.05.012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.49 n.3 2018
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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