Statistical experimental design to assess the influence of enzymes of nematophagous fungi versus helminths

• Autor(a) principal: Braga, Fabio Ribeiro
• Data de Publicação: 2014
• Outros Autores: Soares, Filippe Elias de Freitas, Araujo, Juliana Milani, Fonseca, Leandro Abreu da, Hiura, Emy, Gava, Maylla Garschagen, Vieira, Fernanda Toledo, Paz, Jeanne Saraiva da, Carvalho, Lorendane Milena de, Faccini, João Victor, Queiroz, José Humberto de, Araújo, Jackson Victor
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1016/j.rvsc.2014.09.005; http://www.locus.ufv.br/handle/123456789/18299
• topic: Biological control; Helminths; Plackett–Burman; Fungal enzymes

The present work used Plackett–Burman experimental design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett–Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. westeri and trichostrongylides larvae, and P. fastosum eggs. All tested enzymes had a significant effect (P < 0.05) on the destruction of S. westeri larvae. Furthermore, only VC4 and AC001 proteases showed a significant effect (P < 0.05) on the destruction of trichostrongylides larvae. On the other hand, chitinases of VC4 showed the highest significance (P < 0.05) on the destruction of P. fastosum eggs. It is proposed that statistical planning for the use of enzymes derived from nematophagous fungi is a viable way to elucidate some questions about their mechanism of action.