Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500160 |
Resumo: | Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production. |
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Brazilian Journal of Microbiology |
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Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareolComparative proteomicHyphozyma roseonigraSclareolSclareol glycolAmbroxideAbstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production.Sociedade Brasileira de Microbiologia2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500160Brazilian Journal of Microbiology v.49 suppl.1 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2018.04.001info:eu-repo/semantics/openAccessWang,XiuwenZhang,XiaohuaYao,QingshouHua,DongliangQin,Jiayangeng2018-11-29T00:00:00Zoai:scielo:S1517-83822018000500160Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-11-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol |
title |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol |
spellingShingle |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol Wang,Xiuwen Comparative proteomic Hyphozyma roseonigra Sclareol Sclareol glycol Ambroxide |
title_short |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol |
title_full |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol |
title_fullStr |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol |
title_full_unstemmed |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol |
title_sort |
Comparative proteomic analyses of Hyphozyma roseonigra ATCC 20624 in response to sclareol |
author |
Wang,Xiuwen |
author_facet |
Wang,Xiuwen Zhang,Xiaohua Yao,Qingshou Hua,Dongliang Qin,Jiayang |
author_role |
author |
author2 |
Zhang,Xiaohua Yao,Qingshou Hua,Dongliang Qin,Jiayang |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Wang,Xiuwen Zhang,Xiaohua Yao,Qingshou Hua,Dongliang Qin,Jiayang |
dc.subject.por.fl_str_mv |
Comparative proteomic Hyphozyma roseonigra Sclareol Sclareol glycol Ambroxide |
topic |
Comparative proteomic Hyphozyma roseonigra Sclareol Sclareol glycol Ambroxide |
description |
Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of sclareol on cells and the proteins involved in sclareol metabolism. Comparative proteomic analyses were conducted on the strain H. roseonigra ATCC 20624 by using sclareol or glucose as the sole carbon source. A total of 79 up-regulated protein spots with a >2.0-fold difference in abundance on 2-D gels under sclareol stress conditions were collected for further identification. Seventy spots were successfully identified and finally integrated into 30 proteins. The up-regulated proteins under sclareol stress are involved in carbon metabolism; and nitrogen metabolism; and replication, transcription, and translation processes. Eighteen up-regulated spots were identified as aldehyde dehydrogenases, which indicating that aldehyde dehydrogenases might play an important role in sclareol metabolism. Overall, this study may lay the fundamentals for further cell engineering to improve sclareol glycol production. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500160 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500160 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2018.04.001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.49 suppl.1 2018 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122209886797824 |