Microbiota associated with chronic osteomyelitis of the jaws

Detalhes bibliográficos
Autor(a) principal: Gaetti-Jardim Júnior,Elerson
Data de Publicação: 2010
Outros Autores: Fardin,Angélica Cristiane, Gaetti-Jardim,Ellen Cristina, Castro,Alvimar Lima de, Schweitzer,Christiane Marie, Avila-Campos,Mario Julio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400025
Resumo: Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors.
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spelling Microbiota associated with chronic osteomyelitis of the jawsosteomyelitisbacteriaanaerobesmaxillamandibleChronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors.Sociedade Brasileira de Microbiologia2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400025Brazilian Journal of Microbiology v.41 n.4 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000400025info:eu-repo/semantics/openAccessGaetti-Jardim Júnior,ElersonFardin,Angélica CristianeGaetti-Jardim,Ellen CristinaCastro,Alvimar Lima deSchweitzer,Christiane MarieAvila-Campos,Mario Julioeng2010-08-09T00:00:00Zoai:scielo:S1517-83822010000400025Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-08-09T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Microbiota associated with chronic osteomyelitis of the jaws
title Microbiota associated with chronic osteomyelitis of the jaws
spellingShingle Microbiota associated with chronic osteomyelitis of the jaws
Gaetti-Jardim Júnior,Elerson
osteomyelitis
bacteria
anaerobes
maxilla
mandible
title_short Microbiota associated with chronic osteomyelitis of the jaws
title_full Microbiota associated with chronic osteomyelitis of the jaws
title_fullStr Microbiota associated with chronic osteomyelitis of the jaws
title_full_unstemmed Microbiota associated with chronic osteomyelitis of the jaws
title_sort Microbiota associated with chronic osteomyelitis of the jaws
author Gaetti-Jardim Júnior,Elerson
author_facet Gaetti-Jardim Júnior,Elerson
Fardin,Angélica Cristiane
Gaetti-Jardim,Ellen Cristina
Castro,Alvimar Lima de
Schweitzer,Christiane Marie
Avila-Campos,Mario Julio
author_role author
author2 Fardin,Angélica Cristiane
Gaetti-Jardim,Ellen Cristina
Castro,Alvimar Lima de
Schweitzer,Christiane Marie
Avila-Campos,Mario Julio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gaetti-Jardim Júnior,Elerson
Fardin,Angélica Cristiane
Gaetti-Jardim,Ellen Cristina
Castro,Alvimar Lima de
Schweitzer,Christiane Marie
Avila-Campos,Mario Julio
dc.subject.por.fl_str_mv osteomyelitis
bacteria
anaerobes
maxilla
mandible
topic osteomyelitis
bacteria
anaerobes
maxilla
mandible
description Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822010000400025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.41 n.4 2010
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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