Microbiota associated with chronic osteomyelitis of the jaws

Detalhes bibliográficos
Autor(a) principal: Jardim Júnior, Élerson Gaetti [UNESP]
Data de Publicação: 2010
Outros Autores: Fardin, Angélica Cristiane [UNESP], Gaetti-Jardim, Ellen Cristina [UNESP], Castro, Alvimar Lima de [UNESP], Schweitzer, Christiane Marie, Avila-Campos, Mario Julio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1517-83822010000400025
http://hdl.handle.net/11449/15663
Resumo: Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors.
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spelling Microbiota associated with chronic osteomyelitis of the jawsosteomyelitisbacteriaanaerobesmaxillamandibleChronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Faculdade de Odontologia de Araçatuba Departamento de PatologiaUniversidade Federal do ABC Centro de Matemática, Computação e CogniçãoUniversidade de São Paulo Departamento de Microbiologia Laboratório de AnaeróbiosUniversidade Estadual Paulista Faculdade de Odontologia de Araçatuba Departamento de PatologiaFAPESP: 02/07371-0FAPESP: 07/54851-0Sociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Universidade Federal do ABC (UFABC)Universidade de São Paulo (USP)Jardim Júnior, Élerson Gaetti [UNESP]Fardin, Angélica Cristiane [UNESP]Gaetti-Jardim, Ellen Cristina [UNESP]Castro, Alvimar Lima de [UNESP]Schweitzer, Christiane MarieAvila-Campos, Mario Julio2013-09-30T18:31:13Z2014-05-20T13:44:41Z2013-09-30T18:31:13Z2014-05-20T13:44:41Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1056-1064application/pdfhttp://dx.doi.org/10.1590/S1517-83822010000400025Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 41, n. 4, p. 1056-1064, 2010.1517-8382http://hdl.handle.net/11449/1566310.1590/S1517-83822010000400025S1517-83822010000400025WOS:000286432800025S1517-83822010000400025.pdf4605992512582464803955752674113200011553552103270000-0001-9561-8281SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-09-19T18:57:27Zoai:repositorio.unesp.br:11449/15663Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:57:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Microbiota associated with chronic osteomyelitis of the jaws
title Microbiota associated with chronic osteomyelitis of the jaws
spellingShingle Microbiota associated with chronic osteomyelitis of the jaws
Jardim Júnior, Élerson Gaetti [UNESP]
osteomyelitis
bacteria
anaerobes
maxilla
mandible
title_short Microbiota associated with chronic osteomyelitis of the jaws
title_full Microbiota associated with chronic osteomyelitis of the jaws
title_fullStr Microbiota associated with chronic osteomyelitis of the jaws
title_full_unstemmed Microbiota associated with chronic osteomyelitis of the jaws
title_sort Microbiota associated with chronic osteomyelitis of the jaws
author Jardim Júnior, Élerson Gaetti [UNESP]
author_facet Jardim Júnior, Élerson Gaetti [UNESP]
Fardin, Angélica Cristiane [UNESP]
Gaetti-Jardim, Ellen Cristina [UNESP]
Castro, Alvimar Lima de [UNESP]
Schweitzer, Christiane Marie
Avila-Campos, Mario Julio
author_role author
author2 Fardin, Angélica Cristiane [UNESP]
Gaetti-Jardim, Ellen Cristina [UNESP]
Castro, Alvimar Lima de [UNESP]
Schweitzer, Christiane Marie
Avila-Campos, Mario Julio
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do ABC (UFABC)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Jardim Júnior, Élerson Gaetti [UNESP]
Fardin, Angélica Cristiane [UNESP]
Gaetti-Jardim, Ellen Cristina [UNESP]
Castro, Alvimar Lima de [UNESP]
Schweitzer, Christiane Marie
Avila-Campos, Mario Julio
dc.subject.por.fl_str_mv osteomyelitis
bacteria
anaerobes
maxilla
mandible
topic osteomyelitis
bacteria
anaerobes
maxilla
mandible
description Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-01
2013-09-30T18:31:13Z
2013-09-30T18:31:13Z
2014-05-20T13:44:41Z
2014-05-20T13:44:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822010000400025
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 41, n. 4, p. 1056-1064, 2010.
1517-8382
http://hdl.handle.net/11449/15663
10.1590/S1517-83822010000400025
S1517-83822010000400025
WOS:000286432800025
S1517-83822010000400025.pdf
4605992512582464
8039557526741132
0001155355210327
0000-0001-9561-8281
url http://dx.doi.org/10.1590/S1517-83822010000400025
http://hdl.handle.net/11449/15663
identifier_str_mv Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 41, n. 4, p. 1056-1064, 2010.
1517-8382
10.1590/S1517-83822010000400025
S1517-83822010000400025
WOS:000286432800025
S1517-83822010000400025.pdf
4605992512582464
8039557526741132
0001155355210327
0000-0001-9561-8281
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Microbiology
1.810
0,630
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1056-1064
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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