Microbiota associated with chronic osteomyelitis of the jaws
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822010000400025 http://hdl.handle.net/11449/15663 |
Resumo: | Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors. |
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Microbiota associated with chronic osteomyelitis of the jawsosteomyelitisbacteriaanaerobesmaxillamandibleChronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Faculdade de Odontologia de Araçatuba Departamento de PatologiaUniversidade Federal do ABC Centro de Matemática, Computação e CogniçãoUniversidade de São Paulo Departamento de Microbiologia Laboratório de AnaeróbiosUniversidade Estadual Paulista Faculdade de Odontologia de Araçatuba Departamento de PatologiaFAPESP: 02/07371-0FAPESP: 07/54851-0Sociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Universidade Federal do ABC (UFABC)Universidade de São Paulo (USP)Jardim Júnior, Élerson Gaetti [UNESP]Fardin, Angélica Cristiane [UNESP]Gaetti-Jardim, Ellen Cristina [UNESP]Castro, Alvimar Lima de [UNESP]Schweitzer, Christiane MarieAvila-Campos, Mario Julio2013-09-30T18:31:13Z2014-05-20T13:44:41Z2013-09-30T18:31:13Z2014-05-20T13:44:41Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1056-1064application/pdfhttp://dx.doi.org/10.1590/S1517-83822010000400025Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 41, n. 4, p. 1056-1064, 2010.1517-8382http://hdl.handle.net/11449/1566310.1590/S1517-83822010000400025S1517-83822010000400025WOS:000286432800025S1517-83822010000400025.pdf4605992512582464803955752674113200011553552103270000-0001-9561-8281SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-09-19T18:57:27Zoai:repositorio.unesp.br:11449/15663Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:57:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Microbiota associated with chronic osteomyelitis of the jaws |
title |
Microbiota associated with chronic osteomyelitis of the jaws |
spellingShingle |
Microbiota associated with chronic osteomyelitis of the jaws Jardim Júnior, Élerson Gaetti [UNESP] osteomyelitis bacteria anaerobes maxilla mandible |
title_short |
Microbiota associated with chronic osteomyelitis of the jaws |
title_full |
Microbiota associated with chronic osteomyelitis of the jaws |
title_fullStr |
Microbiota associated with chronic osteomyelitis of the jaws |
title_full_unstemmed |
Microbiota associated with chronic osteomyelitis of the jaws |
title_sort |
Microbiota associated with chronic osteomyelitis of the jaws |
author |
Jardim Júnior, Élerson Gaetti [UNESP] |
author_facet |
Jardim Júnior, Élerson Gaetti [UNESP] Fardin, Angélica Cristiane [UNESP] Gaetti-Jardim, Ellen Cristina [UNESP] Castro, Alvimar Lima de [UNESP] Schweitzer, Christiane Marie Avila-Campos, Mario Julio |
author_role |
author |
author2 |
Fardin, Angélica Cristiane [UNESP] Gaetti-Jardim, Ellen Cristina [UNESP] Castro, Alvimar Lima de [UNESP] Schweitzer, Christiane Marie Avila-Campos, Mario Julio |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do ABC (UFABC) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Jardim Júnior, Élerson Gaetti [UNESP] Fardin, Angélica Cristiane [UNESP] Gaetti-Jardim, Ellen Cristina [UNESP] Castro, Alvimar Lima de [UNESP] Schweitzer, Christiane Marie Avila-Campos, Mario Julio |
dc.subject.por.fl_str_mv |
osteomyelitis bacteria anaerobes maxilla mandible |
topic |
osteomyelitis bacteria anaerobes maxilla mandible |
description |
Chronic osteomyelitis of maxilla and mandible is rare in industrialized countries and its occurrence in developing countries is associated with trauma and surgery, and its microbial etiology has not been studied thoroughly. The aim of this investigation was to evaluate the microbiota associated with osteomyelitis of mandible or maxilla from some Brazilian patients. After clinical and radiographic evaluation, samples of bone sequestra, purulent secretion, and biopsies of granulomatous tissues from twenty-two patients with chronic osteomyelitis of mandible and maxilla were cultivated and submitted for pathogen detection by using a PCR method. Each patient harbored a single lesion. Bacterial isolation was performed on fastidious anaerobe agar supplemented with hemin, menadione and horse blood for anaerobes; and on tryptic soy agar supplemented with yeast extract and horse blood for facultative bacteria and aerobes. Plates were incubated in anaerobiosis and aerobiosis, at 37ºC for 14 and 3 days, respectively. Bacteria were cultivated from twelve patient samples; and genera Actinomyces, Fusobacterium, Parvimonas, and Staphylococcus were the most frequent. By PCR, bacterial DNA was detected from sixteen patient samples. The results suggest that cases of chronic osteomyelitis of the jaws are usually mixed anaerobic infections, reinforcing the concept that osteomyelitis of the jaws are mainly related to microorganisms from the oral environment, and periapical and periodontal infections may act as predisposing factors. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 2013-09-30T18:31:13Z 2013-09-30T18:31:13Z 2014-05-20T13:44:41Z 2014-05-20T13:44:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822010000400025 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 41, n. 4, p. 1056-1064, 2010. 1517-8382 http://hdl.handle.net/11449/15663 10.1590/S1517-83822010000400025 S1517-83822010000400025 WOS:000286432800025 S1517-83822010000400025.pdf 4605992512582464 8039557526741132 0001155355210327 0000-0001-9561-8281 |
url |
http://dx.doi.org/10.1590/S1517-83822010000400025 http://hdl.handle.net/11449/15663 |
identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 41, n. 4, p. 1056-1064, 2010. 1517-8382 10.1590/S1517-83822010000400025 S1517-83822010000400025 WOS:000286432800025 S1517-83822010000400025.pdf 4605992512582464 8039557526741132 0001155355210327 0000-0001-9561-8281 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1056-1064 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546474044129280 |