Effect of the Kozak sequence on seroconversion of mice immunized with a DNA vaccine against swine colibacilosis

• Autor(a) principal: Michelon,André
• Data de Publicação: 2003
• Outros Autores: Michelon,Marcelo, Simionatto,Simone, Lagranha,Valeska Lizzi, Conceição,Fabrício Rochedo, Vaz,Eliana Knackfus, Cerqueira,Gustavo Maia, Dellagostin,Odir Antonio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500029
• topic: swine colibacilosis; DNA vaccine; faeC; Kozak sequence

The neonatal diarrhea in swine caused by enterotoxigenic Escherichia coli (ETEC) is responsible for high mortality and low growth rate in pigs and it is mainly dependent on the capacity of E. coli to attach to the surface of the small intestine, a property mediated by fimbria. In this study the faeC gene, which codes for the minor fimbrial subunit of E. coli K88ab, was cloned in the eukaryotic expression vector pcDNA3, associated or not to the Kozak sequence. Plasmid DNA of the two versions of the vaccine candidate was inoculated in mice by the intramuscular route, in two doses, at 0 and 21 days. The animals that received the DNA vaccine containing faeC associated to the Kozak sequence presented seroconversion significantly higher (P<0.05) than the one vaccinated with pcDNA3/faeC without the Kozak sequence.