Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Detalhes bibliográficos
Autor(a) principal: Miyata,Marcelo
Data de Publicação: 2011
Outros Autores: Santos,Adolfo Carlos Barreto, Mendes,Natália Helena, Cunha,Eunice Atsuko, Melo,Fernando Augusto Fiúza de, Leite,Clarice Queico Fujimura
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045
Resumo: We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
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spelling Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotypingDNA extractionMycobacterium tuberculosisthermolysisCTABWe report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.Sociedade Brasileira de Microbiologia2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045Brazilian Journal of Microbiology v.42 n.2 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000200045info:eu-repo/semantics/openAccessMiyata,MarceloSantos,Adolfo Carlos BarretoMendes,Natália HelenaCunha,Eunice AtsukoMelo,Fernando Augusto Fiúza deLeite,Clarice Queico Fujimuraeng2011-06-06T00:00:00Zoai:scielo:S1517-83822011000200045Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-06-06T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
spellingShingle Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
Miyata,Marcelo
DNA extraction
Mycobacterium tuberculosis
thermolysis
CTAB
title_short Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_full Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_fullStr Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_full_unstemmed Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_sort Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
author Miyata,Marcelo
author_facet Miyata,Marcelo
Santos,Adolfo Carlos Barreto
Mendes,Natália Helena
Cunha,Eunice Atsuko
Melo,Fernando Augusto Fiúza de
Leite,Clarice Queico Fujimura
author_role author
author2 Santos,Adolfo Carlos Barreto
Mendes,Natália Helena
Cunha,Eunice Atsuko
Melo,Fernando Augusto Fiúza de
Leite,Clarice Queico Fujimura
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Miyata,Marcelo
Santos,Adolfo Carlos Barreto
Mendes,Natália Helena
Cunha,Eunice Atsuko
Melo,Fernando Augusto Fiúza de
Leite,Clarice Queico Fujimura
dc.subject.por.fl_str_mv DNA extraction
Mycobacterium tuberculosis
thermolysis
CTAB
topic DNA extraction
Mycobacterium tuberculosis
thermolysis
CTAB
description We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822011000200045
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.42 n.2 2011
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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