Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Detalhes bibliográficos
Autor(a) principal: Miyata, Marcelo [UNESP]
Data de Publicação: 2011
Outros Autores: Santos, Adolfo Carlos Barreto [UNESP], Mendes, Natália Helena, Cunha, Eunice Atsuko, Melo, Fernando Augusto Fiúza de, Leite, Clarice Queico Fujimura
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1517-83822011000200045
http://hdl.handle.net/11449/7935
Resumo: We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
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spelling Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotypingDNA extractionMycobacterium tuberculosisthermolysisCTABWe report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Faculdade de Ciências FarmacêuticasLaboratório Central de Saúde PúblicaInstituto Clemente FerreiraUniversidade Estadual Paulista Faculdade de Ciências FarmacêuticasSociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Laboratório Central de Saúde PúblicaInstituto Clemente FerreiraMiyata, Marcelo [UNESP]Santos, Adolfo Carlos Barreto [UNESP]Mendes, Natália HelenaCunha, Eunice AtsukoMelo, Fernando Augusto Fiúza deLeite, Clarice Queico Fujimura2014-05-20T13:25:05Z2014-05-20T13:25:05Z2011-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article774-777application/pdfhttp://dx.doi.org/10.1590/S1517-83822011000200045Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 774-777, 2011.1517-8382http://hdl.handle.net/11449/793510.1590/S1517-83822011000200045S1517-83822011000200045WOS:000291614000045S1517-83822011000200045.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2023-10-01T06:05:09Zoai:repositorio.unesp.br:11449/7935Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:40:03.371579Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
spellingShingle Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
Miyata, Marcelo [UNESP]
DNA extraction
Mycobacterium tuberculosis
thermolysis
CTAB
title_short Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_full Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_fullStr Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_full_unstemmed Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_sort Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
author Miyata, Marcelo [UNESP]
author_facet Miyata, Marcelo [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Mendes, Natália Helena
Cunha, Eunice Atsuko
Melo, Fernando Augusto Fiúza de
Leite, Clarice Queico Fujimura
author_role author
author2 Santos, Adolfo Carlos Barreto [UNESP]
Mendes, Natália Helena
Cunha, Eunice Atsuko
Melo, Fernando Augusto Fiúza de
Leite, Clarice Queico Fujimura
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Laboratório Central de Saúde Pública
Instituto Clemente Ferreira
dc.contributor.author.fl_str_mv Miyata, Marcelo [UNESP]
Santos, Adolfo Carlos Barreto [UNESP]
Mendes, Natália Helena
Cunha, Eunice Atsuko
Melo, Fernando Augusto Fiúza de
Leite, Clarice Queico Fujimura
dc.subject.por.fl_str_mv DNA extraction
Mycobacterium tuberculosis
thermolysis
CTAB
topic DNA extraction
Mycobacterium tuberculosis
thermolysis
CTAB
description We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
2014-05-20T13:25:05Z
2014-05-20T13:25:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822011000200045
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 774-777, 2011.
1517-8382
http://hdl.handle.net/11449/7935
10.1590/S1517-83822011000200045
S1517-83822011000200045
WOS:000291614000045
S1517-83822011000200045.pdf
url http://dx.doi.org/10.1590/S1517-83822011000200045
http://hdl.handle.net/11449/7935
identifier_str_mv Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 42, n. 2, p. 774-777, 2011.
1517-8382
10.1590/S1517-83822011000200045
S1517-83822011000200045
WOS:000291614000045
S1517-83822011000200045.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Microbiology
1.810
0,630
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 774-777
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128262773145600

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