Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi

Detalhes bibliográficos
Autor(a) principal: Brilhante,Raimunda Sâmia Nogueira
Data de Publicação: 2016
Outros Autores: Caetano,Érica Pacheco, Lima,Rita Amanda Chaves de, Marques,Francisca Jakelyne de Farias, Castelo-Branco,Débora de Souza Collares Maia, Melo,Charlline Vládia Silva de, Guedes,Glaucia Morgana de Melo, Oliveira,Jonathas Sales de, Camargo,Zoilo Pires de, Moreira,José Luciano Bezerra, Monteiro,André Jalles, Bandeira,Tereza de Jesus Pinheiro Gomes, Cordeiro,Rossana de Aguiar, Rocha,Marcos Fábio Gadelha, Sidrim,José Júlio Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400917
Resumo: Abstract This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n = 22) and Histoplasma capsulatum in both filamentous (n = 40) and yeast phases (n = 13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 µg/mL, 20-2860 µg/mL, and 40-1420 µg/mL, respectively for terpinen-4-ol; 250-4000 µg/mL, 30-2000 µg/mL, and 10-1000 µg/mL, respectively, for tyrosol; and 0.48-7.8 µg/mL, 0.25-16 µg/mL, and 0.125-4 µg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules.
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spelling Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungiDimorphic fungiTerpinen-4-olTyrosolβ-LapachoneInhibitory effectAbstract This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n = 22) and Histoplasma capsulatum in both filamentous (n = 40) and yeast phases (n = 13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 µg/mL, 20-2860 µg/mL, and 40-1420 µg/mL, respectively for terpinen-4-ol; 250-4000 µg/mL, 30-2000 µg/mL, and 10-1000 µg/mL, respectively, for tyrosol; and 0.48-7.8 µg/mL, 0.25-16 µg/mL, and 0.125-4 µg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules.Sociedade Brasileira de Microbiologia2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400917Brazilian Journal of Microbiology v.47 n.4 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.07.015info:eu-repo/semantics/openAccessBrilhante,Raimunda Sâmia NogueiraCaetano,Érica PachecoLima,Rita Amanda Chaves deMarques,Francisca Jakelyne de FariasCastelo-Branco,Débora de Souza Collares MaiaMelo,Charlline Vládia Silva deGuedes,Glaucia Morgana de MeloOliveira,Jonathas Sales deCamargo,Zoilo Pires deMoreira,José Luciano BezerraMonteiro,André JallesBandeira,Tereza de Jesus Pinheiro GomesCordeiro,Rossana de AguiarRocha,Marcos Fábio GadelhaSidrim,José Júlio Costaeng2016-11-21T00:00:00Zoai:scielo:S1517-83822016000400917Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-11-21T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
title Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
spellingShingle Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
Brilhante,Raimunda Sâmia Nogueira
Dimorphic fungi
Terpinen-4-ol
Tyrosol
β-Lapachone
Inhibitory effect
title_short Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
title_full Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
title_fullStr Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
title_full_unstemmed Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
title_sort Terpinen-4-ol, tyrosol, and β-lapachone as potential antifungals against dimorphic fungi
author Brilhante,Raimunda Sâmia Nogueira
author_facet Brilhante,Raimunda Sâmia Nogueira
Caetano,Érica Pacheco
Lima,Rita Amanda Chaves de
Marques,Francisca Jakelyne de Farias
Castelo-Branco,Débora de Souza Collares Maia
Melo,Charlline Vládia Silva de
Guedes,Glaucia Morgana de Melo
Oliveira,Jonathas Sales de
Camargo,Zoilo Pires de
Moreira,José Luciano Bezerra
Monteiro,André Jalles
Bandeira,Tereza de Jesus Pinheiro Gomes
Cordeiro,Rossana de Aguiar
Rocha,Marcos Fábio Gadelha
Sidrim,José Júlio Costa
author_role author
author2 Caetano,Érica Pacheco
Lima,Rita Amanda Chaves de
Marques,Francisca Jakelyne de Farias
Castelo-Branco,Débora de Souza Collares Maia
Melo,Charlline Vládia Silva de
Guedes,Glaucia Morgana de Melo
Oliveira,Jonathas Sales de
Camargo,Zoilo Pires de
Moreira,José Luciano Bezerra
Monteiro,André Jalles
Bandeira,Tereza de Jesus Pinheiro Gomes
Cordeiro,Rossana de Aguiar
Rocha,Marcos Fábio Gadelha
Sidrim,José Júlio Costa
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brilhante,Raimunda Sâmia Nogueira
Caetano,Érica Pacheco
Lima,Rita Amanda Chaves de
Marques,Francisca Jakelyne de Farias
Castelo-Branco,Débora de Souza Collares Maia
Melo,Charlline Vládia Silva de
Guedes,Glaucia Morgana de Melo
Oliveira,Jonathas Sales de
Camargo,Zoilo Pires de
Moreira,José Luciano Bezerra
Monteiro,André Jalles
Bandeira,Tereza de Jesus Pinheiro Gomes
Cordeiro,Rossana de Aguiar
Rocha,Marcos Fábio Gadelha
Sidrim,José Júlio Costa
dc.subject.por.fl_str_mv Dimorphic fungi
Terpinen-4-ol
Tyrosol
β-Lapachone
Inhibitory effect
topic Dimorphic fungi
Terpinen-4-ol
Tyrosol
β-Lapachone
Inhibitory effect
description Abstract This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains of Coccidioides posadasii in filamentous phase (n = 22) and Histoplasma capsulatum in both filamentous (n = 40) and yeast phases (n = 13), using the broth dilution methods as described by the Clinical and Laboratory Standards Institute, to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of these compounds. The mechanisms of action of these compounds were also investigated by analyzing their effect on cell membrane permeability and ergosterol synthesis. The MIC and MFCf these compounds against C. posadasii, mycelial H. capsulatum, and yeast-like H. capsulatum, were in the following ranges: 350-5720 µg/mL, 20-2860 µg/mL, and 40-1420 µg/mL, respectively for terpinen-4-ol; 250-4000 µg/mL, 30-2000 µg/mL, and 10-1000 µg/mL, respectively, for tyrosol; and 0.48-7.8 µg/mL, 0.25-16 µg/mL, and 0.125-4 µg/mL, respectively for β-lapachone. These compounds showed a decrease in MIC when the samples were subjected to osmotic stress, suggesting that the compounds acted on the fungal membrane. All the compounds were able to reduce the ergosterol content of the fungal strains. Finally, tyrosol was able to cause a leakage of intracellular molecules.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400917
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2016.07.015
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.47 n.4 2016
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
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instname_str Sociedade Brasileira de Microbiologia (SBM)
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reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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