Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism

Detalhes bibliográficos
Autor(a) principal: Jiang,Yun-Gang
Data de Publicação: 2014
Outros Autores: Wang,Chu-Yan, Jin,Chao, Jia,Jun-Qiang, Guo,Xijie, Zhang,Guo-Zheng, Gui,Zhong-Zheng
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048
Resumo: DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.
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spelling Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganismmulberryDNJ ProductionmicroorganismsfermentationDNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.Sociedade Brasileira de Microbiologia2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048Brazilian Journal of Microbiology v.45 n.2 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000200048info:eu-repo/semantics/openAccessJiang,Yun-GangWang,Chu-YanJin,ChaoJia,Jun-QiangGuo,XijieZhang,Guo-ZhengGui,Zhong-Zhengeng2014-09-30T00:00:00Zoai:scielo:S1517-83822014000200048Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-09-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
title Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
spellingShingle Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
Jiang,Yun-Gang
mulberry
DNJ Production
microorganisms
fermentation
title_short Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
title_full Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
title_fullStr Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
title_full_unstemmed Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
title_sort Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
author Jiang,Yun-Gang
author_facet Jiang,Yun-Gang
Wang,Chu-Yan
Jin,Chao
Jia,Jun-Qiang
Guo,Xijie
Zhang,Guo-Zheng
Gui,Zhong-Zheng
author_role author
author2 Wang,Chu-Yan
Jin,Chao
Jia,Jun-Qiang
Guo,Xijie
Zhang,Guo-Zheng
Gui,Zhong-Zheng
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jiang,Yun-Gang
Wang,Chu-Yan
Jin,Chao
Jia,Jun-Qiang
Guo,Xijie
Zhang,Guo-Zheng
Gui,Zhong-Zheng
dc.subject.por.fl_str_mv mulberry
DNJ Production
microorganisms
fermentation
topic mulberry
DNJ Production
microorganisms
fermentation
description DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000200048
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.2 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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