Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048 |
Resumo: | DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf. |
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Brazilian Journal of Microbiology |
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Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganismmulberryDNJ ProductionmicroorganismsfermentationDNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf.Sociedade Brasileira de Microbiologia2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048Brazilian Journal of Microbiology v.45 n.2 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000200048info:eu-repo/semantics/openAccessJiang,Yun-GangWang,Chu-YanJin,ChaoJia,Jun-QiangGuo,XijieZhang,Guo-ZhengGui,Zhong-Zhengeng2014-09-30T00:00:00Zoai:scielo:S1517-83822014000200048Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-09-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism |
title |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism |
spellingShingle |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism Jiang,Yun-Gang mulberry DNJ Production microorganisms fermentation |
title_short |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism |
title_full |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism |
title_fullStr |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism |
title_full_unstemmed |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism |
title_sort |
Improved 1-Deoxynojirimycin (DNJ) production in mulberry leaves fermented by microorganism |
author |
Jiang,Yun-Gang |
author_facet |
Jiang,Yun-Gang Wang,Chu-Yan Jin,Chao Jia,Jun-Qiang Guo,Xijie Zhang,Guo-Zheng Gui,Zhong-Zheng |
author_role |
author |
author2 |
Wang,Chu-Yan Jin,Chao Jia,Jun-Qiang Guo,Xijie Zhang,Guo-Zheng Gui,Zhong-Zheng |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Jiang,Yun-Gang Wang,Chu-Yan Jin,Chao Jia,Jun-Qiang Guo,Xijie Zhang,Guo-Zheng Gui,Zhong-Zheng |
dc.subject.por.fl_str_mv |
mulberry DNJ Production microorganisms fermentation |
topic |
mulberry DNJ Production microorganisms fermentation |
description |
DNJ, an inhibitor of α-glucosidase, is used to suppress the elevation of postprandial hyperglycemia. In this study, we focus on screening an appropriate microorganism for performing fermentation to improve DNJ content in mulberry leaf. Results showed that Ganoderma lucidum was selected from 8 species and shown to be the most effective in improvement of DNJ production from mulberry leaves through fermentation. Based on single factor and three factor influence level tests by following the Plackett-Burman design, the optimum extraction yield was analyzed by response surface methodology (RSM). The extracted DNJ was determined by reverse-phase high performance liquid chromatograph equipped with fluorescence detector (HPLC-FD). The results of RSM showed that the optimal condition for mulberry fermentation was defined as pH 6.97, potassium nitrate content 0.81% and inoculums volume 2 mL. The extraction efficiency reached to 0.548% in maximum which is 2.74 fold of those in mulberry leaf. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200048 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822014000200048 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.45 n.2 2014 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122206560714752 |