Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287 |
Resumo: | Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes. |
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Brazilian Journal of Microbiology |
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Preliminary studies of new strains of Trametes sp. from Argentina for laccase production abilityWhite rot fungiTrametesLaccaseManganese peroxidaseCu2+ responseAbstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes.Sociedade Brasileira de Microbiologia2016-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287Brazilian Journal of Microbiology v.47 n.2 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.01.002info:eu-repo/semantics/openAccessFonseca,María IsabelTejerina,Marcos RaúlSawostjanik-Afanasiuk,Silvana SoledadGiorgio,Ernesto MartinBarchuk,Mónica LucreciaZapata,Pedro DaríoVillalba,Laura Lidiaeng2016-05-03T00:00:00Zoai:scielo:S1517-83822016000200287Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-05-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability |
title |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability |
spellingShingle |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability Fonseca,María Isabel White rot fungi Trametes Laccase Manganese peroxidase Cu2+ response |
title_short |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability |
title_full |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability |
title_fullStr |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability |
title_full_unstemmed |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability |
title_sort |
Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability |
author |
Fonseca,María Isabel |
author_facet |
Fonseca,María Isabel Tejerina,Marcos Raúl Sawostjanik-Afanasiuk,Silvana Soledad Giorgio,Ernesto Martin Barchuk,Mónica Lucrecia Zapata,Pedro Darío Villalba,Laura Lidia |
author_role |
author |
author2 |
Tejerina,Marcos Raúl Sawostjanik-Afanasiuk,Silvana Soledad Giorgio,Ernesto Martin Barchuk,Mónica Lucrecia Zapata,Pedro Darío Villalba,Laura Lidia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fonseca,María Isabel Tejerina,Marcos Raúl Sawostjanik-Afanasiuk,Silvana Soledad Giorgio,Ernesto Martin Barchuk,Mónica Lucrecia Zapata,Pedro Darío Villalba,Laura Lidia |
dc.subject.por.fl_str_mv |
White rot fungi Trametes Laccase Manganese peroxidase Cu2+ response |
topic |
White rot fungi Trametes Laccase Manganese peroxidase Cu2+ response |
description |
Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu2+. Strain B showed the greatest response to Cu2+ addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.01.002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.2 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122208339099648 |